Detection and isolation of intestinal muscle relaxant substances from the root of Taverniera abyssinica A. Rich.

Animals Guinea Pigs Rabbits Plant Extracts / pharmacology Intestines Fabaceae Duodenum Ileum Muscle, Smooth Muscle Contraction
Afrormosin Bioassay Formononetin Muscle strip Smooth muscle Tectorigenin

Journal

Journal of ethnopharmacology
ISSN: 1872-7573
Titre abrégé: J Ethnopharmacol
Pays: Ireland
ID NLM: 7903310

Informations de publication

Date de publication:
10 Aug 2023
Historique:
received: 18 02 2023
revised: 11 04 2023
accepted: 12 04 2023
medline: 8 5 2023
pubmed: 15 4 2023
entrez: 14 4 2023
Statut: ppublish

Résumé

In Ethiopian traditional medicine the root of Taverniera abyssinica A.Rich is known as a remedy for sudden gastrointestinal cramping and fever. In this study we have isolated and identified the bioactive principle of Taverniera abyssinica that exerts effects on isolated smooth muscle tissues of the rabbit duodenum and guinea-pig ileum. To isolate and purify the bioactive principle from the root of Taverniera abyssinica A.Rich by bioassay-guided fractionation, HPLC purification and masspectrometry, with further investigation of its bioactivity on isolated smooth muscle strips. Roots of Taverniera abyssinica A.Rich extracted in 75% methanol/water were fractioned with a reverse phase column and then subjected to HPLC purification. Each fraction collected from the HPLC was tested for its bioactivity using electric field stimulation-evoked contractions of the rabbit duodenum and guinea-pig ileum. Finally, detailed structural analysis of the fraction displaying significant bioactivity was made by mass spectrometry. Through bioassay-guided fractionation and HPLC purification the bioactive fractions were identified. These were tested for bioactivity on isolated smooth muscle strips which showed about 80% inhibition of contractions evoked by electric field stimulation. These compounds were identified as formononetin, afrormosin and tectorigenin by using masspectrometry applying relevant standards for detection. The traditionally claimed smooth muscle-relaxing effect of the roots of Taverniera abyssinica A.Rich is essentially due the three isolated and purified the two isoflavones formononetin, afrormosin as well as the metoxyisoflavone tectorigenin, along with possibly other not yet purified bioactive substances, however with similar smooth muscle-relaxing properties.

Identifiants

DOI: 10.1016/j.jep.2023.116498 PMID: 37059252
pubmed: 37059252
pii: S0378-8741(23)00366-5
doi: 10.1016/j.jep.2023.116498
pii:
doi:

Substances chimiques

Plant Extracts 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

116498

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest None of the authors have any conflict of interest to report.

Auteurs

Daniel Seifu (D)

Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Department of Biochemistry, Addis Ababa University, Addis Ababa, Ethiopia; Department of Biochemistry, Division of Biomedical Sciences, School of medicine, University of Global Health Equity, Kigali, Rwanda.

Kristofer F Nilsson (KF)

Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden; Department of Cardiothoracic and Vascular Surgery, Örebro University, Örebro, Sweden.

Rajinder Chawla (R)

Department of Biochemistry, Addis Ababa University, Addis Ababa, Ethiopia.

Solomon Genet (S)

Department of Biochemistry, Addis Ababa University, Addis Ababa, Ethiopia.

Mikael Holst (M)

Department of Medical Sciences, Uppsala University, Uppsala, Sweden.

Asfaw Debella (A)

Ethiopian Public Health Institute, Addis Ababa, Ethiopia.

Per M Hellström (PM)

Department of Medical Sciences, Uppsala University, Uppsala, Sweden. Electronic address: Per.Hellstrom@medsci.uu.se.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Detection and isolation of intestinal muscle...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Cochons d'Inde › Detection and isolation of intestinal muscle...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Lagomorpha › Lapins › Detection and isolation of intestinal muscle...
questionsmedicales.fr › Préparations pharmaceutiques › Extraits de plantes › Detection and isolation of intestinal muscle...
questionsmedicales.fr › Système digestif › Tube digestif › Intestins › Detection and isolation of intestinal muscle...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Fabaceae › Detection and isolation of intestinal muscle...
questionsmedicales.fr › Système digestif › Tube digestif › Tube digestif supérieur › Duodénum › Detection and isolation of intestinal muscle...
questionsmedicales.fr › Système digestif › Tube digestif › Tube digestif inférieur › Iléum › Detection and isolation of intestinal muscle...
questionsmedicales.fr › Tissus › Muscles › Muscles lisses › Detection and isolation of intestinal muscle...
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