Mannan oligosaccharides alleviate oxidative injury in the head kidney and spleen in grass carp (Ctenopharyngodon idella) via the Nrf2 signaling pathway after Aeromonas hydrophila infection.
Antioxidant
Apoptosis
Functional organs
Grass carp
Mannan oligosaccharides
Journal
Journal of animal science and biotechnology
ISSN: 1674-9782
Titre abrégé: J Anim Sci Biotechnol
Pays: England
ID NLM: 101581293
Informations de publication
Date de publication:
15 Apr 2023
15 Apr 2023
Historique:
received:
16
09
2022
accepted:
31
01
2023
medline:
15
4
2023
entrez:
14
4
2023
pubmed:
15
4
2023
Statut:
epublish
Résumé
Mannan oligosaccharides (MOS) are recommended as aquaculture additives owing to their excellent antioxidant properties. In the present study, we examined the effects of dietary MOS on the head kidney and spleen of grass carp (Ctenopharyngodon idella) with Aeromonas hydrophila infection. A total of 540 grass carp were used for the study. They were administered six gradient dosages of the MOS diet (0, 200, 400, 600, 800, and 1,000 mg/kg) for 60 d. Subsequently, we performed a 14-day Aeromonas hydrophila challenge experiment. The antioxidant capacity of the head kidney and spleen were examined using spectrophotometry, DNA fragmentation, qRT-PCR, and Western blotting. After infection with Aeromonas hydrophila, 400-600 mg/kg MOS supplementation decreased the levels of reactive oxygen species, protein carbonyl, and malonaldehyde and increased the levels of anti-superoxide anion, anti-hydroxyl radical, and glutathione in the head kidney and spleen of grass carp. The activities of copper-zinc superoxide dismutase, manganese superoxide dismutase, catalase, glutathione S-transferase, glutathione reductase, and glutathione peroxidase were also enhanced by supplementation with 400-600 mg/kg MOS. Furthermore, the expression of most antioxidant enzymes and their corresponding genes increased significantly with supplementation of 200-800 mg/kg MOS. mRNA and protein levels of nuclear factor erythroid 2-related factor 2 also increased following supplementation with 400-600 mg/kg MOS. In addition, supplementation with 400-600 mg/kg MOS reduced excessive apoptosis by inhibiting the death receptor pathway and mitochondrial pathway processes. Based on the quadratic regression analysis of the above biomarkers (reactive oxygen species, malondialdehyde, and protein carbonyl) of oxidative damage in the head kidney and spleen of on-growing grass carp, the recommended MOS supplementation is 575.21, 557.58, 531.86, 597.35, 570.16, and 553.80 mg/kg, respectively. Collectively, MOS supplementation could alleviate oxidative injury in the head kidney and spleen of grass carp infected with Aeromonas hydrophila.
Sections du résumé
BACKGROUND
BACKGROUND
Mannan oligosaccharides (MOS) are recommended as aquaculture additives owing to their excellent antioxidant properties. In the present study, we examined the effects of dietary MOS on the head kidney and spleen of grass carp (Ctenopharyngodon idella) with Aeromonas hydrophila infection.
METHODS
METHODS
A total of 540 grass carp were used for the study. They were administered six gradient dosages of the MOS diet (0, 200, 400, 600, 800, and 1,000 mg/kg) for 60 d. Subsequently, we performed a 14-day Aeromonas hydrophila challenge experiment. The antioxidant capacity of the head kidney and spleen were examined using spectrophotometry, DNA fragmentation, qRT-PCR, and Western blotting.
RESULTS
RESULTS
After infection with Aeromonas hydrophila, 400-600 mg/kg MOS supplementation decreased the levels of reactive oxygen species, protein carbonyl, and malonaldehyde and increased the levels of anti-superoxide anion, anti-hydroxyl radical, and glutathione in the head kidney and spleen of grass carp. The activities of copper-zinc superoxide dismutase, manganese superoxide dismutase, catalase, glutathione S-transferase, glutathione reductase, and glutathione peroxidase were also enhanced by supplementation with 400-600 mg/kg MOS. Furthermore, the expression of most antioxidant enzymes and their corresponding genes increased significantly with supplementation of 200-800 mg/kg MOS. mRNA and protein levels of nuclear factor erythroid 2-related factor 2 also increased following supplementation with 400-600 mg/kg MOS. In addition, supplementation with 400-600 mg/kg MOS reduced excessive apoptosis by inhibiting the death receptor pathway and mitochondrial pathway processes.
CONCLUSIONS
CONCLUSIONS
Based on the quadratic regression analysis of the above biomarkers (reactive oxygen species, malondialdehyde, and protein carbonyl) of oxidative damage in the head kidney and spleen of on-growing grass carp, the recommended MOS supplementation is 575.21, 557.58, 531.86, 597.35, 570.16, and 553.80 mg/kg, respectively. Collectively, MOS supplementation could alleviate oxidative injury in the head kidney and spleen of grass carp infected with Aeromonas hydrophila.
Identifiants
pubmed: 37060042
doi: 10.1186/s40104-023-00844-1
pii: 10.1186/s40104-023-00844-1
pmc: PMC10105433
doi:
Types de publication
Journal Article
Langues
eng
Pagination
58Subventions
Organisme : the National Key R&D Program of China
ID : 2019YFD0900200
Organisme : the National Key R&D Program of China
ID : 2018YFD0900400
Organisme : CARS (CARS-45)
ID : CARS (CARS-45)
Organisme : National Outstanding Youth Science Fund Project of National Natural Science Foundation of China
ID : 31922086
Organisme : the Young Top-Notch Talent Support Program
ID : the Young Top-Notch Talent Support Program
Organisme : Sichuan Province Science and Technology Support Program
ID : 2019YFN0036
Informations de copyright
© 2023. The Author(s).
Références
Genet Mol Res. 2016 Jun 03;15(2):
pubmed: 27323135
Eur J Med Chem. 2015 Jun 5;97:55-74
pubmed: 25942353
Dev Comp Immunol. 2001 Oct-Dec;25(8-9):827-39
pubmed: 11602198
Fish Shellfish Immunol. 2014 Feb;36(2):525-44
pubmed: 24412165
Mol Cell. 2002 Mar;9(3):459-70
pubmed: 11931755
J S Afr Vet Assoc. 2005 Mar;76(1):40-2
pubmed: 15900900
Anim Nutr. 2019 Dec;5(4):366-372
pubmed: 31890913
Environ Pollut. 2021 Aug 1;282:117010
pubmed: 33848913
Blood. 2003 Mar 15;101(6):2393-400
pubmed: 12433687
Am J Pathol. 2000 Nov;157(5):1415-30
pubmed: 11073801
Mutat Res. 1999 Mar 8;424(1-2):83-95
pubmed: 10064852
Front Immunol. 2020 Feb 05;11:114
pubmed: 32117265
Anim Nutr. 2020 Dec;6(4):457-466
pubmed: 33364462
Nat Rev Mol Cell Biol. 2004 Nov;5(11):897-907
pubmed: 15520809
Ecotoxicol Environ Saf. 2016 Apr;126:30-37
pubmed: 26707186
Bull Environ Contam Toxicol. 1998 Feb;60(2):300-5
pubmed: 9470993
Fish Shellfish Immunol. 2020 Nov;106:596-608
pubmed: 32841685
Anim Nutr. 2021 Jun;7(2):556-568
pubmed: 34258445
Genes Dev. 2001 Nov 15;15(22):2922-33
pubmed: 11711427
Mol Cell Biol. 2004 Aug;24(16):7130-9
pubmed: 15282312
Cell. 2010 Mar 19;140(6):951-951.e2
pubmed: 20303882
Pathophysiology. 2000 Sep;7(3):153-163
pubmed: 10996508
Fish Shellfish Immunol. 2013 Jun;34(6):1395-403
pubmed: 23481213
Fish Shellfish Immunol. 2019 Mar;86:35-45
pubmed: 30339845
Free Radic Res. 2004 Dec;38(12):1281-7
pubmed: 15763952
Aquat Toxicol. 2015 Jan;158:125-37
pubmed: 25461751
Fish Shellfish Immunol. 2011 Mar;30(3):830-5
pubmed: 21255653
J Anim Physiol Anim Nutr (Berl). 2010 Dec;94(6):747-56
pubmed: 20050948
Antioxidants (Basel). 2022 Apr 20;11(5):
pubmed: 35624670
Inorg Chem. 2010 May 3;49(9):4036-45
pubmed: 20380391
Sci Total Environ. 2020 Mar 10;707:135630
pubmed: 31784173
J Biol Chem. 2004 May 7;279(19):20451-60
pubmed: 14993216
Mech Ageing Dev. 2006 Apr;127(4):384-90
pubmed: 16442588
Clin Biochem. 2010 Oct;43(15):1220-4
pubmed: 20691171
Aquat Toxicol. 2015 Jul;164:61-71
pubmed: 25917764
Fish Shellfish Immunol. 2007 Nov;23(5):969-81
pubmed: 17766145
Arch Gerontol Geriatr. 2012 Mar-Apr;54(2):e1-7
pubmed: 21784539
Fish Shellfish Immunol. 2017 Apr;63:103-126
pubmed: 28192254
Fish Shellfish Immunol. 2018 May;76:272-278
pubmed: 29510254
Experientia. 1985 Feb 15;41(2):152-8
pubmed: 3972063
Free Radic Biol Med. 2009 Nov 1;47(9):1304-9
pubmed: 19666107
Ecotoxicol Environ Saf. 2009 Jan;72(1):199-205
pubmed: 18308394
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Dev Comp Immunol. 2013 Apr;39(4):447-55
pubmed: 23219904
Curr Opin Cell Biol. 2015 Apr;33:8-13
pubmed: 25305438
Blood. 2015 Jun 18;125(25):3851-9
pubmed: 25900980
Chem Biol Interact. 2007 Jan 5;165(1):59-75
pubmed: 17145051
Int J Biol Macromol. 2018 Feb;107(Pt A):755-761
pubmed: 28928062
Front Immunol. 2021 Oct 18;12:742107
pubmed: 34733280
J Anim Physiol Anim Nutr (Berl). 2012 Aug;96(4):591-9
pubmed: 21707781
Anim Nutr. 2020 Mar;6(1):69-79
pubmed: 32211531
World Allergy Organ J. 2012 Jan;5(1):9-19
pubmed: 23268465
J Toxicol Environ Health B Crit Rev. 2006 Jan-Feb;9(1):27-39
pubmed: 16393868