Protocol to measure calcium spikes in cardiomyocytes obtained from human pluripotent stem cells using a ready-to-use media.
Cell Biology
Cell Culture
Cell Differentiation
Flow Cytometry/Mass Cytometry
Microscopy
Stem Cells
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
13 Apr 2023
13 Apr 2023
Historique:
received:
29
12
2022
revised:
22
02
2023
accepted:
28
03
2023
entrez:
15
4
2023
pubmed:
16
4
2023
medline:
16
4
2023
Statut:
aheadofprint
Résumé
The derivation of cardiomyocytes from human pluripotent stem cells (hPSCs) is a powerful tool to investigate early cardiogenesis and model diseases in vitro. Here, we present an optimized protocol to obtain contracting hPSCs-derived cardiomyocytes using a ready-to-use kit. We describe steps for hPSC culture and differentiation to cardiomyocytes including the identification of key parameters such as starting cell confluency and temperature. We then detail immunofluorescence, flow cytometry, and the quantification of cardiomyocytes' calcium spikes using live imaging. For complete details on the use and execution of this protocol, please refer to Astro et al.
Identifiants
pubmed: 37060558
pii: S2666-1667(23)00210-1
doi: 10.1016/j.xpro.2023.102252
pmc: PMC10140149
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
102252Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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