Semi-automated and efficient parallel SELEX of aptamers for multiple targets.

Humans Aptamers, Nucleotide / chemistry SELEX Aptamer Technique / methods Reproducibility of Results Flow Cytometry

Journal

Analytical methods : advancing methods and applications
ISSN: 1759-9679
Titre abrégé: Anal Methods
Pays: England
ID NLM: 101519733

Informations de publication

Date de publication:
27 04 2023
Historique:
medline: 1 5 2023
pubmed: 18 4 2023
entrez: 17 4 2023
Statut: epublish

Résumé

In this study, we constructed and optimized a semi-automatic instrument to perform aptamer SELEX targeting multiple proteins simultaneously. Our work provides a simple SELEX platform characterized by real-time feedback, which is time efficient and can reduce human intervention. A number of aptamers were rapidly screened by this method. Moreover, the binding affinities of these aptamers were verified by various methods, including SPR and flow cytometry, which supports the applicability and reliability of our newly established aptamer SELEX system.

Identifiants

DOI: 10.1039/d3ay00367a PMID: 37066673
pubmed: 37066673
doi: 10.1039/d3ay00367a
doi:

Substances chimiques

Aptamers, Nucleotide 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2039-2043

Auteurs

Meng Jiang (M)

School of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255049, China. biometrysdut@163.com.

Xiaona Fang (X)

The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China. lzf@ustc.edu.cn.

Han Diao (H)

College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China. lzf@ustc.edu.cn.

Shaokang Lv (S)

College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou 310014, China.
The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China. lzf@ustc.edu.cn.

Zheng Zhang (Z)

The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China. lzf@ustc.edu.cn.

Xiang Zhang (X)

The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China. lzf@ustc.edu.cn.

Zhiwei Chen (Z)

School of Life Sciences and Medicine, Shandong University of Technology, Zibo, 255049, China. biometrysdut@163.com.
Institute of Food and Nutrition Science, Shandong University of Technology, Zibo, 255049, P. R. China.
School of Agriculture Engineering and Food Science, Shandong University of Technology, Zibo, 255049, P. R. China.
ORCID: 0009-0003-2190-5634

Zhaofeng Luo (Z)

The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, Hangzhou, Zhejiang 310022, China. lzf@ustc.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Semi-automated and efficient parallel SELEX of...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Aptamères nucléotidiques › Semi-automated and efficient parallel SELEX of...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Techniques de chimie combinatoire › Technique SELEX › Semi-automated and efficient parallel SELEX of...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Semi-automated and efficient parallel SELEX of...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en flux › Semi-automated and efficient parallel SELEX of...