Programmable DNA Hydrogel Provides Suitable Microenvironment for Enhancing TSPCS Therapy in Healing of Tendinopathy.
DNA hydrogel
achilles tendinopathy
extracellular matrix
tendon stem/progenitor cells
Journal
Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338
Informations de publication
Date de publication:
08 2023
08 2023
Historique:
revised:
17
03
2023
received:
21
11
2022
medline:
10
8
2023
pubmed:
18
4
2023
entrez:
17
4
2023
Statut:
ppublish
Résumé
Tendon stem/progenitor cells (TSPCs) therapy is a promising strategy for enhancing cell matrix and collagen synthesis, and regulating the metabolism of the tendon microenvironment during tendon injury repair. Nevertheless, the barren microenvironment and gliding shear of tendon cause insufficient nutrition supply, damage, and aggregation of injected TSPCs around tendon tissues, which severely hinders their clinical application in tendinopathy. In this study, a TSPCs delivery system is developed by encapsulating TSPCs within a DNA hydrogel (TSPCs-Gel) as the DNA hydrogel offers an excellent artificial extracellular matrix (ECM) microenvironment by providing nutrition for proliferation and protection against shear forces. This delivery method restricts TSPCs to the tendons, significantly extending their retention time. It is also found that TSPCs-Gel injections can promote the healing of rat tendinopathy in vivo, where cross-sectional area and load to failure of injured tendons in rats are significantly improved compared to the free TSPCs treatment group at 8 weeks. Furthermore, the potential healing mechanism of TSPCs-Gel is investigated by RNA-sequencing to identify a series of potential gene and signaling pathway targets for further clinical treatment strategies. These findings suggest the potential pathways of using DNA hydrogels as artificial ECMs to promote cell proliferation and protect TSPCs in TSPC therapy.
Identifiants
pubmed: 37066733
doi: 10.1002/smll.202207231
doi:
Substances chimiques
Hydrogels
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2207231Informations de copyright
© 2023 The Authors. Small published by Wiley-VCH GmbH.
Références
C. N. van Dijk, M. N. van Sterkenburg, J. I. Wiegerinck, J. Karlsson, N. Maffulli, Knee Surg. Sports Traumatol. Arthrosc. 2011, 19, 835.
N. Maffulli, J. Wong, L. C. Almekinders, Clin. Sports Med. 2003, 22, 675.
a) U. M. Kujala, S. Sarna, J. Kaprio, Clin. J. Sport Med. 2005, 15, 133;
b) C. Hopkins, S. C. Fu, E. Chua, X. Hu, C. Rolf, V. M. Mattila, L. Qin, P. S. Yung, K. M. Chan, Asia Pac. J. Sports Med. Arthrosc. Rehabil. Technol. 2016, 4, 9.
A. van der Vlist, M. Winters, A. Weir, C. Ardern, N. Welton, D. Caldwell, J. Verhaar, R. de Vos, Br. J. Sports Med. 2021, 55, 249.
a) Y. Cho, H. Kim, D. Kang, H. Kim, N. Lee, J. Yun, Y. Kim, K. Lee, J. Kim, H. Kim, Y. Hwang, C. Jo, J. Kim, Sci. Adv. 2021, 7, eabg6069;
b) T. Harvey, S. Flamenco, C. Fan, Nat. Cell Biol. 2019, 21, 1490;
c) Y. Bi, D. Ehirchiou, T. Kilts, C. Inkson, M. Embree, W. Sonoyama, L. Li, A. Leet, B. Seo, L. Zhang, S. Shi, M. Young, Nat. Med. 2007, 13, 1219.
a) Y. Wang, G. He, Y. Guo, H. Tang, Y. Shi, X. Bian, M. Zhu, X. Kang, M. Zhou, J. Lyu, M. Yang, M. Mu, F. Lai, K. Lu, W. Chen, B. Zhou, J. Zhang, K. Tang, J. Cell Mol. Med. 2019, 23, 5475;
b) J. Chen, Q. Yu, B. Wu, Z. Lin, N. J. Pavlos, J. Xu, H. Ouyang, A. Wang, M. H. Zheng, Tissue Eng., Part A 2011, 17, 2037.
a) M. Gomez-Florit, C. Labrador-Rached, R. Domingues, M. Gomes, Adv. Drug Delivery Rev. 2022, 185, 114299;
b) H. Shaw, M. Benjamin, Scand. J. Med. Sci. Sports 2007, 17, 303.
X. Yan, B. Yang, Y. Chen, Y. Song, J. Ye, Y. Pan, B. Zhou, Y. Wang, F. Mao, Y. Dong, Adv. Mater. 2021, 33, 2104758.
a) Y. Wei, K. Wang, S. Luo, F. Li, X. Zuo, C. Fan, Q. Li, Small 2022, 18, 2107640;
b) Y. S. Zhang, A. Khademhosseini, Science 2017, 356, eaaf3627;
c) A. Chrisnandy, D. Blondel, S. Rezakhani, N. Broguiere, M. P. Lutolf, Nat. Mater. 2022, 21, 479;
d) K. Zhang, Q. Feng, Z. Fang, L. Gu, L. Bian, Chem. Rev. 2021, 121, 11149;
e) X. Ji, D. Guo, J. Ma, M. Yin, Y. Yu, C. Liu, Y. Zhou, J. Sun, Q. Li, N. Chen, Adv. Mater. 2021, 33, 2100949.
J. Wu, B. R. Liyarita, H. Zhu, M. Liu, X. Hu, F. Shao, ACS Appl. Mater. Interfaces 2021, 13, 49705.
a) G. Jose, K. Shalumon, J.-P. Chen, Curr. Med. Chem. 2020, 27, 2734;
b) A. K. Azab, B. Orkin, V. Doviner, A. Nissan, M. Klein, M. Srebnik, A. Rubinstein, J. Controlled Release 2006, 111, 281;
c) P. Lou, S. Liu, Y. Wang, C. Pan, X. Xu, M. Zhao, G. Liao, G. Yang, Y. Yuan, L. Li, Acta Biomater. 2021, 135, 100;
d) J. Han, Y. Guo, H. Wang, K. Zhang, D. Yang, J. Am. Chem. Soc. 2021, 143, 19486;
e) Z. Wang, W. Li, L. Gou, Y. Zhou, G. Peng, J. Zhang, J. Liu, R. Li, H. Ni, W. Zhang, T. Cao, Q. Cao, H. Su, Y. Han, N. Tong, X. Fu, E. Ilegems, Y. Lu, P. Berggren, X. Zheng, C. Wang, Adv. Healthcare Mater. 2022, e2200782;
f) S. Liu, Q. Yan, S. Cao, L. Wang, S.-H. Luo, M. Lv, ACS Appl. Mater. Interfaces 2022, 21, 41809.
a) F. Mo, K. Jiang, D. Zhao, Y. Wang, J. Song, W. Tan, Adv. Drug Delivery Rev. 2021, 168, 79;
b) Y. Dong, C. Yao, Y. Zhu, L. Yang, D. Luo, D. Yang, Chem. Rev. 2020, 120, 9420;
c) N. Ren, R. Sun, K. Xia, Q. Zhang, W. Li, F. Wang, X. Zhang, Z. Ge, L. Wang, C. Fan, ACS Appl. Mater. Interfaces 2019, 11, 26704;
d) W. Li, C. Wang, Z. Wang, L. Gou, Y. Zhou, G. Peng, M. Zhu, J. Zhang, R. Li, H. Ni, ACS Appl. Mater. Interfaces 2022, 14, 6287.
T. Yuan, Y. Shao, X. Zhou, Q. Liu, Z. Zhu, B. Zhou, Y. Dong, N. Stephanopoulos, S. Gui, H. Yan, Adv. Mater. 2021, 33, 2102428.
a) Y. Shao, H. Jia, T. Cao, D. Liu, Acc. Chem. Res. 2017, 50, 659;
b) Y. Xing, E. Cheng, Y. Yang, P. Chen, T. Zhang, Y. Sun, Z. Yang, D. Liu, Adv. Mater. 2011, 23, 1117.
a) P. P. Lui, O. T. Wong, Y. W. Lee, Cytotherapy 2016, 18, 99;
b) T. M. Kraus, F. B. Imhoff, G. Wexel, A. Wolf, D. Hirsch, L. Lenz, U. Stöckle, S. Buchmann, T. Tischer, A. B. Imhoff, S. Milz, M. Anton, S. Vogt, J. Bone Joint Surg. Am. 2014, 96, 761.
U. G. Longo, M. Ronga, N. Maffulli, Sports Med. Arthrosc. Rev. 2018, 26, 16.
R. Yang, Y. Zheng, Y. Zhang, G. Li, Y. Xu, Y. Xu, C. Zhuang, P. Yu, L. Deng, W. Cui, Adv. Healthcare Mater. 2022, 11, 2200072.
a) D. Lin, P. Alberton, M. D. Caceres, E. Volkmer, M. Schieker, D. Docheva, Cell Death Dis. 2017, 8, e3116;
b) C. Stoll, T. John, C. Conrad, A. Lohan, S. Hondke, W. Ertel, C. Kaps, M. Endres, M. Sittinger, J. Ringe, G. Schulze-Tanzil, Biomaterials 2011, 32, 4806.
Y. Wang, G. He, H. Tang, Y. Shi, X. Kang, J. Lyu, M. Zhu, M. Zhou, M. Yang, M. Mu, Cell Prolif. 2019, 52, e12650.
Z. Ge, H. Tang, J. Lyu, B. Zhou, M. Yang, K. Tang, W. Chen, Ann. Transl. Med. 2020, 8, 335.
A. Abraham, S. Shah, M. Golman, L. Song, X. Li, I. Kurtaliaj, M. Akbar, N. Millar, Y. Abu-Amer, L. Galatz, S. Thomopoulos, Sci. Transl. Med. 2019, 11, eaav4319.
M. Golman, X. Li, D. Skouteris, A. Abraham, L. Song, Y. Abu-Amer, S. Thomopoulos, Am. J. Sports Med. 2021, 49, 780.
X. Wang, S. Liu, T. Yu, S. An, R. Deng, X. Tan, J. Crane, W. Zhang, D. Pan, M. Wan, A. Carr, X. Cao, Adv. Sci. 2022, 9, e2104469.
A. Subramanian, T. Schilling, Development 2015, 142, 4191.
Z. Ge, H. Tang, W. Chen, Y. Wang, C. Yuan, X. Tao, B. Zhou, K. Tang, J. Orthop. Surg. Res. 2020, 15, 1.
Y. Bu, H. Shen, F. Yang, Y. Yang, X. Wang, D. Wu, ACS Appl. Mater. Interfaces 2017, 9, 2205.
D. Kim, B. Langmead, S. L. Salzberg, Nat. Methods 2015, 12, 357.
M. Pertea, G. M. Pertea, C. M. Antonescu, T.-C. Chang, J. T. Mendell, S. L. Salzberg, Nat. Biotechnol. 2015, 33, 290.