Genetically encoded barcodes for correlative volume electron microscopy.
Journal
Nature biotechnology
ISSN: 1546-1696
Titre abrégé: Nat Biotechnol
Pays: United States
ID NLM: 9604648
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
10
04
2022
accepted:
14
02
2023
pubmed:
18
4
2023
medline:
18
4
2023
entrez:
17
4
2023
Statut:
ppublish
Résumé
While genetically encoded reporters are common for fluorescence microscopy, equivalent multiplexable gene reporters for electron microscopy (EM) are still scarce. Here, by installing a variable number of fixation-stable metal-interacting moieties in the lumen of encapsulin nanocompartments of different sizes, we developed a suite of spherically symmetric and concentric barcodes (EMcapsulins) that are readable by standard EM techniques. Six classes of EMcapsulins could be automatically segmented and differentiated. The coding capacity was further increased by arranging several EMcapsulins into distinct patterns via a set of rigid spacers of variable length. Fluorescent EMcapsulins were expressed to monitor subcellular structures in light and EM. Neuronal expression in Drosophila and mouse brains enabled the automatic identification of genetically defined cells in EM. EMcapsulins are compatible with transmission EM, scanning EM and focused ion beam scanning EM. The expandable palette of genetically controlled EM-readable barcodes can augment anatomical EM images with multiplexed gene expression maps.
Identifiants
pubmed: 37069313
doi: 10.1038/s41587-023-01713-y
pii: 10.1038/s41587-023-01713-y
pmc: PMC10713455
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1734-1745Subventions
Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
ID : ERC-COG 865710
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : LF4D
Informations de copyright
© 2023. The Author(s).
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