Urinary T Cells Identify Renal Antineutrophil Cytoplasmic Antibody-Associated Vasculitis and Predict Prognosis: A Proof of Concept Study.

ANCA TH Treg vasculitis

Journal

Kidney international reports
ISSN: 2468-0249
Titre abrégé: Kidney Int Rep
Pays: United States
ID NLM: 101684752

Informations de publication

Date de publication:
Apr 2023
Historique:
received: 15 08 2022
revised: 19 12 2022
accepted: 05 01 2023
medline: 19 4 2023
entrez: 18 4 2023
pubmed: 19 4 2023
Statut: epublish

Résumé

Necrotizing crescentic glomerulonephritis is a major contributor to morbidity and mortality in Antineutrophil cytoplasmic antibodies (ANCA)-associated vasculitis (AAV). Because therapy relies on immunosuppressive agents with potentially severe adverse effects, a reliable noninvasive biomarker of disease activity is needed to guide treatment. We used flow cytometry to quantify T cell subsets in blood and urine samples from 95 patients with AAV and 8 controls to evaluate their biomarker characteristics. These were compared to soluble markers, monocyte chemoattractant protein-1 (MCP-1), soluble CD163 (sCD163), soluble CD25 (sCD25), and complement C5a (C5a), measured using multiplex analysis. Available kidney biopsies ( Patients with active renal AAV (rAAV) showed significantly higher urinary cell counts than those in remission, or those with extrarenal manifestation, or healthy controls. Urinary T cells showed robust discrimination of disease activity with superior performance compared to MCP-1 and sCD163. Patients whose kidney biopsies had been classified as "crescentic" according to Berden classification showed higher urinary T cell counts. Discordant regulatory T cells (T Urinary T cells reflect the renal inflammatory milieu in AAV and provide further insights into the pathogenesis of this chronic condition. Their promising potential as noninvasive diagnostic and prognostic biomarkers deserves further exploitation.

Identifiants

DOI: 10.1016/j.ekir.2023.01.013 PMID: 37069968 PMC: PMC10105048
pubmed: 37069968
doi: 10.1016/j.ekir.2023.01.013
pii: S2468-0249(23)00014-1
pmc: PMC10105048
doi:

Types de publication

Journal Article

Langues

eng

Pagination

871-883

Informations de copyright

© 2023 International Society of Nephrology. Published by Elsevier Inc.

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Auteurs

Janis Sonnemann (J)

Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Experimental and Clinical Research Center, Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.
Berlin Institute of Health, Berlin, Germany.

Jan Klocke (J)

Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Berlin, Germany.

Markus Bieringer (M)

Department of Nephrology, Helios Klinikum Berlin-Buch, Berlin, Germany.

Anthony Rousselle (A)

Experimental and Clinical Research Center, Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.

Kai-Uwe Eckardt (KU)

Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Berlin, Germany.

Saban Elitok (S)

Department of Nephrology and Endocrinology, Ernst von Bergmann Klinikum, Potsdam, Germany.

Suncica Popovic (S)

Department of Anatomy, Charité-Universitätsmedizin Berlin, Berlin, Germany.

Sebastian Bachmann (S)

Department of Anatomy, Charité-Universitätsmedizin Berlin, Berlin, Germany.

Ralph Kettritz (R)

Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Experimental and Clinical Research Center, Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.

Alan D Salama (AD)

Center for Nephrology, University College London, Royal Free Hospital, London, UK.

Philipp Enghard (P)

Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Deutsches Rheumaforschungszentrum Berlin (DRFZ).

Adrian Schreiber (A)

Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Experimental and Clinical Research Center, Charité-Universitätsmedizin Berlin and Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.

Classifications MeSH