Density separation of petrous bone powders for optimized ancient DNA yields.
Journal
Genome research
ISSN: 1549-5469
Titre abrégé: Genome Res
Pays: United States
ID NLM: 9518021
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
received:
18
01
2023
accepted:
11
04
2023
medline:
17
5
2023
pubmed:
19
4
2023
entrez:
18
4
2023
Statut:
ppublish
Résumé
Density separation is a process routinely used to segregate minerals, organic matter, and even microplastics, from soils and sediments. Here we apply density separation to archaeological bone powders before DNA extraction to increase endogenous DNA recovery relative to a standard control extraction of the same powders. Using nontoxic heavy liquid solutions, we separated powders from the petrous bones of 10 individuals of similar archaeological preservation into eight density intervals (2.15 to 2.45 g/cm
Identifiants
pubmed: 37072186
pii: gr.277714.123
doi: 10.1101/gr.277714.123
pmc: PMC10234301
doi:
Substances chimiques
DNA, Ancient
0
Powders
0
Plastics
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
622-631Informations de copyright
© 2023 Fernandes et al.; Published by Cold Spring Harbor Laboratory Press.
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