Fluorescence recovery after photobleaching: analyses of cyanobacterial phycobilisomes reveal intrinsic fluorescence recovery.
Cyanobacteria
Fluorescence recovery after photobleaching
Intrinsic photoprocess
Mobility
Phycobilisome
Journal
Marine life science & technology
ISSN: 2662-1746
Titre abrégé: Mar Life Sci Technol
Pays: Singapore
ID NLM: 101748793
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
04
08
2020
accepted:
08
04
2021
medline:
1
6
2021
pubmed:
1
6
2021
entrez:
19
04
2023
Statut:
epublish
Résumé
Fluorescence recovery after photobleaching (FRAP) has been used to study the dynamics of the cyanobacterial photosynthesis apparatus since 1997. Fluorescence recovery of cyanobacteria during FRAP was conventionally interpreted as a result of phycobilisome (PBS) diffusion on the surface of the thylakoid membrane. The mechanism of state transition in cyanobacteria has been widely attributed to PBS diffusion. However, in red algae, another PBS-containing group, the intrinsic photoprocess was found to contribute greatly to the fluorescence recovery of PBS, which raises questions concerning the role of FRAP in red algal PBS. Therefore, it is important to re-evaluate the nature of PBS fluorescence recovery in cyanobacteria. In the present study, four cyanobacterial strains with different phenotypes and PBS compositions were used to investigate their FRAP characteristics. Fluorescence recovery of PBS was observed in wholly photobleached cells in all four cyanobacterial strains, in which the contribution of PBS diffusion to the fluorescence recovery was not possible. Moreover, the fluorescence recovered in isolated PBSs and PBS-thylakoid membranes after photobleaching further demonstrated the intrinsic photoprocess nature of fluorescence recovery. These findings suggest that the intrinsic photoprocess contributed to the fluorescence recovery following photobleaching when measured by the FRAP method.
Identifiants
pubmed: 37073268
doi: 10.1007/s42995-021-00104-z
pii: 104
pmc: PMC10077209
doi:
Types de publication
Journal Article
Langues
eng
Pagination
427-433Commentaires et corrections
Type : ErratumIn
Informations de copyright
© Ocean University of China 2021, corrected publication 2021.
Déclaration de conflit d'intérêts
Conflict of interestThe authors declare that they have no conflict of interest.
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