Convection and extracellular matrix binding control interstitial transport of extracellular vesicles.
diffusion
exosome
gradient
integrin binding
spatial concentration
Journal
Journal of extracellular vesicles
ISSN: 2001-3078
Titre abrégé: J Extracell Vesicles
Pays: United States
ID NLM: 101610479
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
revised:
06
03
2023
received:
29
11
2022
accepted:
29
03
2023
medline:
20
4
2023
pubmed:
19
4
2023
entrez:
19
04
2023
Statut:
ppublish
Résumé
Extracellular vesicles (EVs) influence a host of normal and pathophysiological processes in vivo. Compared to soluble mediators, EVs can traffic a wide range of proteins on their surface including extracellular matrix (ECM) binding proteins, and their large size (∼30-150 nm) limits diffusion. We isolated EVs from the MCF10 series-a model human cell line of breast cancer progression-and demonstrated increasing presence of laminin-binding integrins α3β1 and α6β1 on the EVs as the malignant potential of the MCF10 cells increased. Transport of the EVs within a microfluidic device under controlled physiological interstitial flow (0.15-0.75 μm/s) demonstrated that convection was the dominant mechanism of transport. Binding of the EVs to the ECM enhanced the spatial concentration and gradient, which was mitigated by blocking integrins α3β1 and α6β1. Our studies demonstrate that convection and ECM binding are the dominant mechanisms controlling EV interstitial transport and should be leveraged in nanotherapeutic design.
Identifiants
pubmed: 37073802
doi: 10.1002/jev2.12323
pmc: PMC10114097
doi:
Substances chimiques
Laminin
0
Integrin alpha6beta1
0
Integrin alpha3beta1
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12323Subventions
Organisme : NIH HHS
ID : UH3 HL141800
Pays : United States
Organisme : NIH HHS
ID : R21 AI161041
Pays : United States
Organisme : NIH HHS
ID : F31 NS120590
Pays : United States
Organisme : NIH HHS
ID : R01 EB030410
Pays : United States
Organisme : NIH HHS
ID : R01 CA241666
Pays : United States
Organisme : NINDS NIH HHS
ID : F31 NS120590
Pays : United States
Organisme : NIH HHS
ID : T32 OD017863
Pays : United States
Informations de copyright
© 2023 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles.
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