Cyclic pressure induced decellularization of porcine descending aortas.
Adventitial side
Detergent
Intimal side
Porcine aorta
Pressure decellularization
Journal
Journal of materials science. Materials in medicine
ISSN: 1573-4838
Titre abrégé: J Mater Sci Mater Med
Pays: United States
ID NLM: 9013087
Informations de publication
Date de publication:
19 Apr 2023
19 Apr 2023
Historique:
received:
06
04
2022
accepted:
28
03
2023
medline:
21
4
2023
pubmed:
19
4
2023
entrez:
19
04
2023
Statut:
epublish
Résumé
The demand for decellularized xenogeneic tissues used in reconstructive heart surgery has increased over the last decades. Complete decellularization of longer and tubular aortic sections suitable for clinical application has not been achieved so far. The present study aims at analyzing the effect of pressure application on decellularization efficacy of porcine aortas using a device specifically designed for this purpose. Fresh porcine descending aortas of 8 cm length were decellularized using detergents. To increase decellularization efficacy, detergent treatment was combined with pressure application and different treatment schemes. Quantification of penetration depth as well as histological staining, scanning electron microscopy, and tensile strength tests were used to evaluate tissue structure. In general, application of pressure to aortic tissue does neither increase the decellularization success nor the penetration depth of detergents. However, it is of importance from which side of the aorta the pressure is applied. Application of intermittent pressure from the adventitial side does significantly increase the decellularization degree at the intimal side (compared to the reference group), but had no influence on the penetration depth of SDC/SDS at both sides. Although the present setup does not significantly improve the decellularization success of aortas, it is interesting that the application of pressure from the adventitial side leads to improved decellularization of the intimal side. As no adverse effects on tissue structure nor on mechanical properties were observed, optimization of the present protocol may potentially lead to complete decellularization of larger aortic segments.
Identifiants
pubmed: 37074546
doi: 10.1007/s10856-023-06723-5
pii: 10.1007/s10856-023-06723-5
pmc: PMC10115674
doi:
Substances chimiques
Detergents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
19Informations de copyright
© 2023. The Author(s).
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