Quantifying Golgi Apparatus Fragmentation Using Imaging Flow Cytometry.
Golgi
Golgi fragmentation
Golgi structure
Imaging flow cytometry
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
21
4
2023
pubmed:
19
4
2023
entrez:
19
04
2023
Statut:
ppublish
Résumé
Unlike the common conception of the Golgi apparatus as a static organelle, it is, in fact, a dynamic structure, as well as a sensitive sensor for the cellular status. In response to various stimuli, the intact Golgi structure undergoes fragmentation. This fragmentation can yield either partial fragmentation, resulting in several separated chunks, or complete vesiculation of the organelle. These distinct morphologies form the basis of several methods for the quantification of the Golgi status. In this chapter, we describe our imaging flow cytometry-based method for quantifying changes in the Golgi architecture. This method has all the benefits of imaging flow cytometry-namely, it is rapid, high-throughput, and robust-while affording easy implementation and analysis capabilities.
Identifiants
pubmed: 37074663
doi: 10.1007/978-1-0716-3020-4_10
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
173-184Informations de copyright
© 2023. Springer Science+Business Media, LLC, part of Springer Nature.
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