Dynamin-like proteins mediate extracellular vesicle secretion in Mycobacterium tuberculosis.
Mycobacterium tuberculosis
bacterial dynamins
extracellular vesicles
iron response
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
05 06 2023
05 06 2023
Historique:
revised:
14
03
2023
received:
14
06
2022
accepted:
03
04
2023
medline:
6
6
2023
pubmed:
20
4
2023
entrez:
20
04
2023
Statut:
ppublish
Résumé
Mycobacterium tuberculosis (Mtb) secretes extracellular vesicles (EVs) containing a variety of proteins, lipoproteins, and lipoglycans. While emerging evidence suggests that EVs contribute to tuberculosis pathogenesis, the factors and molecular mechanisms involved in mycobacterial EV production have not been identified. In this study, we use a genetic approach to identify Mtb proteins that mediate vesicle release in response to iron limitation and antibiotic exposure. We uncover a critical role for the isoniazid-induced, dynamin-like proteins, IniA and IniC, in mycobacterial EV biogenesis. Further characterization of a Mtb iniA mutant shows that the production of EVs enables intracellular Mtb to export bacterial components into the extracellular environment to communicate with host cells and potentially modulate the immune response. The findings advance our understanding of the biogenesis and functions of mycobacterial EVs and provide an avenue for targeting vesicle production in vivo.
Identifiants
pubmed: 37079766
doi: 10.15252/embr.202255593
pmc: PMC10240201
doi:
Substances chimiques
Isoniazid
V83O1VOZ8L
Dynamins
EC 3.6.5.5
Banques de données
GEO
['GSE143996']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e55593Subventions
Organisme : NIAID NIH HHS
ID : R01 AI162821
Pays : United States
Organisme : NIAID NIH HHS
ID : R03 AI153560
Pays : United States
Informations de copyright
© 2023 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
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