Caveolae and Bin1 form ring-shaped platforms for T-tubule initiation.
T-tubules
caveolae
caveolin 3
caveolinopathies
cell biology
correlative microscopy
human
mouse
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
21 04 2023
21 04 2023
Historique:
received:
12
10
2022
accepted:
20
04
2023
medline:
22
6
2023
pubmed:
21
4
2023
entrez:
21
04
2023
Statut:
epublish
Résumé
Excitation-contraction coupling requires a highly specialized membrane structure, the triad, composed of a plasma membrane invagination, the T-tubule, surrounded by two sarcoplasmic reticulum terminal cisternae. Although the precise mechanisms governing T-tubule biogenesis and triad formation remain largely unknown, studies have shown that caveolae participate in T-tubule formation and mutations of several of their constituents induce muscle weakness and myopathies. Here, we demonstrate that, at the plasma membrane, Bin1 and caveolae composed of caveolin-3 assemble into ring-like structures from which emerge tubes enriched in the dihydropyridine receptor. Bin1 expression lead to the formation of both rings and tubes and we show that Bin1 forms scaffolds on which caveolae accumulate to form the initial T-tubule. Cav3 deficiency caused by either gene silencing or pathogenic mutations results in defective ring formation and perturbed Bin1-mediated tubulation that may explain defective T-tubule organization in mature muscles. Our results uncover new pathophysiological mechanisms that may prove relevant to myopathies caused by Cav3 or Bin1 dysfunction.
Identifiants
pubmed: 37083699
doi: 10.7554/eLife.84139
pii: 84139
pmc: PMC10281672
doi:
pii:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Calcium Channels, L-Type
0
Banques de données
Dryad
['10.5061/dryad.k98sf7m98']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2023, Lemerle et al.
Déclaration de conflit d'intérêts
EL, JL, MB, GM, AB, CL, AM, AC, PA, JV, NR, MB, VM, IM, BC, LP, SV No competing interests declared
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