Using Hyper as a molecular probe to visualize hydrogen peroxide in living plant cells: An updated method.
Journal
Methods in enzymology
ISSN: 1557-7988
Titre abrégé: Methods Enzymol
Pays: United States
ID NLM: 0212271
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
25
4
2023
pubmed:
23
4
2023
entrez:
22
04
2023
Statut:
ppublish
Résumé
Reactive oxygen species (ROS) are highly reactive reduced oxygen molecules that play a myriad of roles in animal and plant cells. In plant cells the production of ROS results from aerobic metabolism during respiration and photosynthesis. Therefore mitochondria, chloroplasts, and peroxisomes constitute an important source of ROS. However, ROS can also be produced in response to many physiological stimuli such as pathogen attack, hormone signaling, abiotic stresses or during cell wall organization and plant morphogenesis. The study of ROS in plant cells has been limited to biochemical assays and use of fluorescent probes, however, the irreversible oxidation of the fluorescent dyes prevents the visualization of dynamic changes. We have previously reported that Hyper 1 is a biosensor for H
Identifiants
pubmed: 37087192
pii: S0076-6879(22)00393-7
doi: 10.1016/bs.mie.2022.09.012
pii:
doi:
Substances chimiques
Molecular Probes
0
Hydrogen Peroxide
BBX060AN9V
Reactive Oxygen Species
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
265-289Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.