Epidemiological and Molecular Characteristics of

Klebsiella pneumoniae blaKPC-2 blaNDM-1 carbapenem resistance illumina sequencing

Journal

Infection and drug resistance
ISSN: 1178-6973
Titre abrégé: Infect Drug Resist
Pays: New Zealand
ID NLM: 101550216

Informations de publication

Date de publication:
2023
Historique:
received: 03 12 2022
accepted: 01 04 2023
medline: 24 4 2023
pubmed: 24 4 2023
entrez: 24 04 2023
Statut: epublish

Résumé

Carbapenem-resistant In this study, Multiplex polymerase chain reaction (PCR) was performed to detect the carbapenem-resistant genes, and the broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of antibacterial drugs. The transferability of carbapenem-resistant phenotypes was examined using filter mating assays. Overall, we used Illumina sequencing to evaluate the epidemiological and molecular characteristics of All strains exhibited resistance to carbapenems and other antibiotics. However, they were still susceptible to polymyxin E. Among them, 18 isolates were positive for These findings suggested that ST11-KL64 CRKP strains are major threats in terms of nosocomial infections in this hospital. Hence, new strategies should be urgently developed to monitor, diagnose, and treat this high-risk CRKP clone.

Identifiants

pubmed: 37090038
doi: 10.2147/IDR.S400138
pii: 400138
pmc: PMC10120834
doi:

Banques de données

figshare
['10.6084/m9.figshare.21360120']

Types de publication

Journal Article

Langues

eng

Pagination

2247-2258

Informations de copyright

© 2023 Rong et al.

Déclaration de conflit d'intérêts

The authors declare no conflicts of interest in this work.

Références

Clin Microbiol Rev. 2006 Jul;19(3):512-30
pubmed: 16847083
Front Microbiol. 2021 Nov 10;12:741093
pubmed: 34858362
Antimicrob Resist Infect Control. 2020 Jan 31;9(1):23
pubmed: 32005246
Infect Drug Resist. 2019 Mar 18;12:641-653
pubmed: 30936727
J Antimicrob Chemother. 2020 Feb 1;75(2):318-326
pubmed: 31665400
Acta Microbiol Immunol Hung. 2018 Mar 1;65(1):107-118
pubmed: 28870092
Diagn Microbiol Infect Dis. 2011 May;70(1):119-23
pubmed: 21398074
Trends Microbiol. 2014 Dec;22(12):686-96
pubmed: 25304194
Emerg Microbes Infect. 2020 Dec;9(1):1771-1779
pubmed: 32689907
Lancet Infect Dis. 2018 Jan;18(1):37-46
pubmed: 28864030
J Glob Antimicrob Resist. 2020 Dec;23:466-470
pubmed: 33212284
Nat Rev Microbiol. 2020 Jun;18(6):344-359
pubmed: 32055025
J Antimicrob Chemother. 2019 Dec 1;74(12):3489-3496
pubmed: 31504589
J Infect. 2019 Mar;78(3):187-199
pubmed: 30503842
Lancet Infect Dis. 2013 Sep;13(9):785-96
pubmed: 23969216
FEMS Microbiol Rev. 2017 May 1;41(3):252-275
pubmed: 28521338
J Glob Antimicrob Resist. 2018 Sep;14:284-286
pubmed: 30103014
Clin Microbiol Infect. 2015 Nov;21(11):1001-7
pubmed: 26166545
Antimicrob Agents Chemother. 2011 Dec;55(12):5493-9
pubmed: 21930889
Ther Clin Risk Manag. 2019 Aug 20;15:1019-1027
pubmed: 31692506
Front Cell Infect Microbiol. 2017 May 16;7:182
pubmed: 28560183
Lancet Infect Dis. 2018 Jan;18(1):24
pubmed: 29102517
Clin Microbiol Rev. 2019 May 15;32(3):
pubmed: 31092506
Emerg Microbes Infect. 2021 Dec;10(1):700-709
pubmed: 33739229
Emerg Microbes Infect. 2021 Dec;10(1):1129-1136
pubmed: 34074225
J Infect Dis. 2020 Mar 16;221(Suppl 2):S206-S214
pubmed: 32176790
Int J Antimicrob Agents. 2007 Mar;29(3):306-10
pubmed: 17276038
Lancet Infect Dis. 2018 Jan;18(1):25
pubmed: 29102518
J Intern Med. 2020 Mar;287(3):283-300
pubmed: 31677303
Emerg Microbes Infect. 2020 Dec;9(1):320-331
pubmed: 32037975
Antimicrob Agents Chemother. 2001 Apr;45(4):1151-61
pubmed: 11257029
Trends Microbiol. 2021 Jan;29(1):65-83
pubmed: 32448764
Clin Microbiol Infect. 2020 Jan;26(1):124.e1-124.e4
pubmed: 31494252
J Microbiol. 2019 Mar;57(3):195-202
pubmed: 30552629
Emerg Infect Dis. 2020 Feb;26(2):289-297
pubmed: 31961299
Microorganisms. 2020 Nov 18;8(11):
pubmed: 33217991
J Med Microbiol. 2018 Sep;67(9):1221-1224
pubmed: 30074471
J Med Microbiol. 2019 Jun;68(6):882-889
pubmed: 31050634
Lancet Infect Dis. 2018 Jan;18(1):2-3
pubmed: 28864028
Virulence. 2013 Feb 15;4(2):107-18
pubmed: 23302790

Auteurs

Fang Rong (F)

Department of General Practice, The Affiliated Hospital of Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.
Graduate School Department of Dalian Medical University, Dalian, Liaoning, People's Republic of China.

Ziyi Liu (Z)

Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.
Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.

Pengbin Yang (P)

Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.
Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.

Feng Wu (F)

Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.

Yu Sun (Y)

Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.

Xuewei Sun (X)

Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.

Jun Zhou (J)

Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, Jiangsu, People's Republic of China.

Classifications MeSH