Morphological Characteristics of Young and Old Murine Hematopoietic Stem Cell Niches, as Modeled
Journal
Scanning
ISSN: 1932-8745
Titre abrégé: Scanning
Pays: England
ID NLM: 7903371
Informations de publication
Date de publication:
2023
2023
Historique:
received:
22
11
2022
revised:
24
03
2023
accepted:
29
03
2023
medline:
26
4
2023
pubmed:
25
4
2023
entrez:
25
04
2023
Statut:
epublish
Résumé
The hematopoietic stem cell (HSC) niche undergoes detrimental changes with age. The molecular differences between young and old niches are well studied and understood; however, young and old niches have not yet been extensively characterized in terms of morphology. In the present work, a 2D stromal model of young and old HSC niches isolated from bone marrow was investigated using light and scanning electron microscopy (SEM) to characterize cell density after one, two, or three weeks of culturing, cell shape, and cell surface morphological features. Our work is aimed at identifying morphological differences between young and old niche cells that could be used to discriminate between their respective murine HSC niches. The results show several age-specific morphological characteristics. The old niches differ from the young ones in terms of lower cell proliferating capacity, increased cell size with a flattened appearance, increased number of adipocytes, and the presence of tunneling nanotubes. In addition, proliferating cell clusters are present in the young niches but not in the old niches. Together, these characteristics could be used as a relatively simple and reliable tool to discriminate between young and old murine HSC niches and as a complementary approach to imaging with specific cellular markers.
Identifiants
pubmed: 37096016
doi: 10.1155/2023/5541050
pmc: PMC10122596
doi:
Substances chimiques
Tunneling Nanotubes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5541050Informations de copyright
Copyright © 2023 Mojca Justin et al.
Déclaration de conflit d'intérêts
The authors declare that there is no conflict of interest regarding the publication of this paper.
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