Adeno-associated virus vector system controlling capsid expression improves viral quantity and quality.
Biotechnology
Virology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
21 Apr 2023
21 Apr 2023
Historique:
received:
01
08
2022
revised:
13
01
2023
accepted:
20
03
2023
medline:
25
4
2023
pubmed:
25
4
2023
entrez:
25
04
2023
Statut:
epublish
Résumé
Adeno-associated virus (AAV) vectors are promising tools for gene therapy. The current AAV vector system produces an abundance of empty capsids that are eliminated before clinical use, leading to increased costs for gene therapy. In the present study, we established an AAV production system that regulates the timing of capsid expression using a tetracycline-dependent promoter. Tetracycline-regulating capsid expression increased viral yield and reduced empty capsids in various serotypes without altering AAV vector infectivity
Identifiants
pubmed: 37096037
doi: 10.1016/j.isci.2023.106487
pii: S2589-0042(23)00564-3
pmc: PMC10122016
doi:
Types de publication
Journal Article
Langues
eng
Pagination
106487Informations de copyright
© 2023 The Author(s).
Déclaration de conflit d'intérêts
T.E. and J.M. are the operating officer and director, respectively, and have treasury stocks in Takara Bio Inc. This work was partly funded by a Joint Research Fund sponsored by Takara Bio Inc. K.O. 1 and TAKARA bio Inc. have applied for the international patent based on present study. 1Kenji Ohba.
Références
Vaccine. 2012 Dec 17;31(1):58-83
pubmed: 23142589
Mol Ther Methods Clin Dev. 2018 May 08;10:1-7
pubmed: 30073177
ACS Nano. 2020 Nov 24;14(11):14262-14283
pubmed: 33073995
Hum Gene Ther. 2014 Nov;25(11):929-41
pubmed: 25072415
Hum Gene Ther. 2017 Apr;28(4):308-313
pubmed: 28335618
Drug Discov Today. 2022 Jan;27(1):17-30
pubmed: 34537333
Hum Gene Ther Methods. 2017 Jun;28(3):101-108
pubmed: 28322595
J Biol Chem. 2021 Jan-Jun;296:100103
pubmed: 33214224
J Nanobiotechnology. 2021 Feb 25;19(1):59
pubmed: 33632278
Future Sci OA. 2021 Jul 14;7(9):FSO744
pubmed: 34737885
Curr Gene Ther. 2014;14(2):86-100
pubmed: 24588706
Intervirology. 2013;56(3):141-65
pubmed: 23594863
Appl Microbiol Biotechnol. 2018 Feb;102(3):1045-1054
pubmed: 29204900
J Virol. 2013 Dec;87(24):13150-60
pubmed: 24067976
Virology. 2017 Oct;510:46-54
pubmed: 28704696
Nat Rev Microbiol. 2004 Feb;2(2):109-22
pubmed: 15043007
Microbiol Mol Biol Rev. 1998 Dec;62(4):1171-90
pubmed: 9841669
Mol Ther Methods Clin Dev. 2017 Dec 01;8:87-104
pubmed: 29326962
Mol Ther Methods Clin Dev. 2017 Jul 24;6:171-182
pubmed: 28828392
J Virol. 1995 Nov;69(11):6880-5
pubmed: 7474103
J Virol. 2022 Jan 26;96(2):e0162921
pubmed: 34705566
Nat Commun. 2021 Mar 12;12(1):1642
pubmed: 33712599
Mol Ther. 2021 Feb 3;29(2):464-488
pubmed: 33309881
Mol Ther Methods Clin Dev. 2018 Jan 08;8:166-180
pubmed: 29687035
Nat Rev Drug Discov. 2019 May;18(5):358-378
pubmed: 30710128
J Virol. 1995 Sep;69(9):5485-96
pubmed: 7636994
J Virol. 2004 Nov;78(21):11841-52
pubmed: 15479826
J Virol. 2011 Dec;85(23):12686-97
pubmed: 21917944
Proc Natl Acad Sci U S A. 2008 Jan 8;105(1):294-9
pubmed: 18172216
Hum Gene Ther Methods. 2012 Feb;23(1):18-28
pubmed: 22428977
J Virol. 1997 Feb;71(2):1079-88
pubmed: 8995628
J Virol. 2001 May;75(9):4150-7
pubmed: 11287564
Annu Rev Virol. 2014 Nov;1(1):427-51
pubmed: 26958729
Mol Ther. 2020 Mar 4;28(3):723-746
pubmed: 31972133
Methods. 2006 Sep;40(1):60-5
pubmed: 16997714
J Virol. 2017 Oct 13;91(21):
pubmed: 28794042
Biotechnol Bioeng. 2021 Nov;118(11):4186-4203
pubmed: 34309017
Mol Ther. 2022 Jun 1;30(6):2122-2129
pubmed: 35390542
Nucleic Acids Res. 2004 May 11;32(8):2652-9
pubmed: 15141036
Nat Immunol. 2021 Nov;22(11):1391-1402
pubmed: 34686865
Nat Rev Drug Discov. 2021 Mar;20(3):173-174
pubmed: 33495615
Nat Biotechnol. 2014 Sep;32(9):874-6
pubmed: 25203033
J Virol. 2017 Apr 28;91(10):
pubmed: 28250132
Hum Mol Genet. 2019 Oct 1;28(R1):R95-R101
pubmed: 31332444
Hum Mol Genet. 2019 Oct 1;28(R1):R102-R107
pubmed: 31238336
Nat Protoc. 2011 Apr;6(4):482-501
pubmed: 21455185
J Virol. 1993 Feb;67(2):997-1005
pubmed: 8380475
Front Immunol. 2021 Oct 29;12:753467
pubmed: 34777364
Mol Ther Methods Clin Dev. 2017 Nov 07;7:146-156
pubmed: 29255740
Proc Natl Acad Sci U S A. 2019 Nov 5;116(45):22485-22490
pubmed: 31570619
Front Immunol. 2020 Apr 17;11:670
pubmed: 32362898
Blood. 2021 Nov 4;138(18):1645-1646
pubmed: 34734998
Nat Med. 2004 Aug;10(8):828-34
pubmed: 15273747
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2020 Jul;12(4):e1613
pubmed: 31960585
J Virol. 1997 Jul;71(7):5236-43
pubmed: 9188591
Biochimie. 2019 Feb;157:38-47
pubmed: 30408502
Sci Adv. 2021 Nov 05;7(45):eabg0811
pubmed: 34730996