Cytotoxicity of dental self-curing resin for a temporary crown: an in vitro study.
Computer-aided design/computer-aided manufacturing block resin
Cytotoxicity test
Methylmethacrylate
Polymethyl methacrylate
Self-curing of dental resin
Journal
Journal of Yeungnam medical science
ISSN: 2799-8010
Titre abrégé: J Yeungnam Med Sci
Pays: Korea (South)
ID NLM: 9918333886606676
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
21
01
2023
accepted:
31
03
2023
medline:
26
4
2023
pubmed:
26
4
2023
entrez:
26
4
2023
Statut:
ppublish
Résumé
Residual monomer tests using high-performance liquid chromatography and cytotoxicity tests were performed to analyze the effect on the oral mucosa of a self-curing resin for provisional crown production. A cytotoxicity test was performed to confirm whether leaked residual monomers directly affected oral mucosal cells. The cytotoxicity of the liquid and solid resin polymers was measured using a water-soluble tetrazolium (WST) test and microplate reader. In the WST assay using a microplate reader, 73.4% of the cells survived at a concentration of 0.2% liquid resin polymer. The cytotoxicity of the liquid resin polymer was low at ≤0.2%. For the solid resins, when 100% of the eluate was used from each specimen, the average cell viability was 91.3% for the solid resin polymer and 100% for the hand-mixed self-curing resin, which is higher than the cell viability standard of 70%. The cytotoxicity of the solid resin polymer was low. Because the polymerization process of the self-curing resin may have harmful effects on the oral mucosa during the second and third stages, the solid resin should be manufactured indirectly using a dental model.
Sections du résumé
BACKGROUND
BACKGROUND
Residual monomer tests using high-performance liquid chromatography and cytotoxicity tests were performed to analyze the effect on the oral mucosa of a self-curing resin for provisional crown production.
METHODS
METHODS
A cytotoxicity test was performed to confirm whether leaked residual monomers directly affected oral mucosal cells. The cytotoxicity of the liquid and solid resin polymers was measured using a water-soluble tetrazolium (WST) test and microplate reader.
RESULTS
RESULTS
In the WST assay using a microplate reader, 73.4% of the cells survived at a concentration of 0.2% liquid resin polymer. The cytotoxicity of the liquid resin polymer was low at ≤0.2%. For the solid resins, when 100% of the eluate was used from each specimen, the average cell viability was 91.3% for the solid resin polymer and 100% for the hand-mixed self-curing resin, which is higher than the cell viability standard of 70%. The cytotoxicity of the solid resin polymer was low.
CONCLUSION
CONCLUSIONS
Because the polymerization process of the self-curing resin may have harmful effects on the oral mucosa during the second and third stages, the solid resin should be manufactured indirectly using a dental model.
Identifiants
pubmed: 37098682
pii: jyms.2023.00080
doi: 10.12701/jyms.2023.00080
doi:
Types de publication
Journal Article
Langues
eng
Pagination
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