A potential histone-chaperone activity for the MIER1 histone deacetylase complex.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
07 07 2023
07 07 2023
Historique:
accepted:
19
04
2023
revised:
10
03
2023
received:
10
08
2022
medline:
10
7
2023
pubmed:
26
4
2023
entrez:
26
4
2023
Statut:
ppublish
Résumé
Histone deacetylases 1 and 2 (HDAC1/2) serve as the catalytic subunit of six distinct families of nuclear complexes. These complexes repress gene transcription through removing acetyl groups from lysine residues in histone tails. In addition to the deacetylase subunit, these complexes typically contain transcription factor and/or chromatin binding activities. The MIER:HDAC complex has hitherto been poorly characterized. Here, we show that MIER1 unexpectedly co-purifies with an H2A:H2B histone dimer. We show that MIER1 is also able to bind a complete histone octamer. Intriguingly, we found that a larger MIER1:HDAC1:BAHD1:C1QBP complex additionally co-purifies with an intact nucleosome on which H3K27 is either di- or tri-methylated. Together this suggests that the MIER1 complex acts downstream of PRC2 to expand regions of repressed chromatin and could potentially deposit histone octamer onto nucleosome-depleted regions of DNA.
Identifiants
pubmed: 37099381
pii: 7143237
doi: 10.1093/nar/gkad294
pmc: PMC10325919
doi:
Substances chimiques
Chromatin
0
Histone Deacetylases
EC 3.5.1.98
Histones
0
Multiprotein Complexes
0
Nucleosomes
0
Transcription Factors
0
MIER1 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6006-6019Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
PLoS Genet. 2016 Mar 03;12(3):e1005898
pubmed: 26938916
Sci Rep. 2018 Apr 18;8(1):6198
pubmed: 29670170
Nat Struct Mol Biol. 2016 Apr;23(4):309-16
pubmed: 26974126
Cold Spring Harb Perspect Biol. 2016 Mar 01;8(3):a018754
pubmed: 26931326
PLoS One. 2017 Jan 3;12(1):e0169338
pubmed: 28046085
EMBO J. 1999 Feb 15;18(4):1014-24
pubmed: 10022843
EMBO Rep. 2008 Jun;9(6):555-62
pubmed: 18451879
J Mol Biol. 2007 Sep 21;372(3):774-97
pubmed: 17681537
J Biol Chem. 1997 Oct 10;272(41):25591-5
pubmed: 9325278
Mol Cell Proteomics. 2016 Nov;15(11):3450-3460
pubmed: 27634302
BMC Res Notes. 2008 Aug 22;1:68
pubmed: 18721470
Clin Immunol Immunopathol. 1997 Jul;84(1):17-26
pubmed: 9191880
Mol Cell. 2014 Feb 6;53(3):498-505
pubmed: 24507717
Mol Cell Biol. 2005 Aug;25(16):6990-7004
pubmed: 16055712
Trends Pharmacol Sci. 2017 Apr;38(4):363-377
pubmed: 28139258
Proc Natl Acad Sci U S A. 2015 Jul 14;112(28):E3669-78
pubmed: 26124119
Proc Natl Acad Sci U S A. 2009 Aug 18;106(33):13826-31
pubmed: 19666599
Biochem J. 2020 Sep 18;477(17):3367-3386
pubmed: 32941645
Elife. 2020 Jun 05;9:
pubmed: 32501215
Cold Spring Harb Perspect Biol. 2014 Apr 01;6(4):a018713
pubmed: 24691964
Nucleic Acids Res. 2018 Aug 21;46(14):7138-7152
pubmed: 29905837
Nat Genet. 2020 Dec;52(12):1384-1396
pubmed: 33139953
Genome Res. 2019 Jun;29(6):978-987
pubmed: 31123082
Mol Cell. 2021 Jun 17;81(12):2669-2681.e9
pubmed: 33894155
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
Mol Cell Proteomics. 2013 Nov;12(11):3199-209
pubmed: 23924515
Nat Protoc. 2007;2(4):933-8
pubmed: 17446892
Science. 2011 Nov 18;334(6058):977-82
pubmed: 22096199
Cell Rep. 2020 Feb 25;30(8):2699-2711.e8
pubmed: 32101746
Nat Biotechnol. 2011 Mar;29(3):255-65
pubmed: 21258344
Nucleic Acids Res. 2021 May 7;49(8):4441-4455
pubmed: 33823544
Nature. 2012 Mar 07;484(7392):115-9
pubmed: 22398447
Nat Commun. 2020 Jun 26;11(1):3252
pubmed: 32591534
FEBS Lett. 1999 Mar 5;446(1):189-93
pubmed: 10100640
Genes Dev. 2001 Mar 15;15(6):710-23
pubmed: 11274056
Sci Adv. 2022 Jul 29;8(30):eabo0517
pubmed: 35895815
Nat Struct Mol Biol. 2008 Aug;15(8):868-9
pubmed: 18641662
J Exp Med. 1994 Jun 1;179(6):1809-21
pubmed: 8195709
Nat Commun. 2019 Jul 1;10(1):2894
pubmed: 31263106
Nucleic Acids Res. 2020 Dec 16;48(22):12972-12982
pubmed: 33264408
J Mol Biol. 1998 Feb 13;276(1):19-42
pubmed: 9514715
Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3572-7
pubmed: 10097078
Nature. 2014 Jan 30;505(7485):648-53
pubmed: 24463511
Nature. 2017 May 25;545(7655):505-509
pubmed: 28514442
Cell Res. 2005 Mar;15(3):183-6
pubmed: 15780180