A low-cost device for cryoanesthesia of neonatal rodents.
Cryoanesthesia
Neonates
Peltier
Rodents
Stereotaxic surgery
Thermoelectric device
Journal
HardwareX
ISSN: 2468-0672
Titre abrégé: HardwareX
Pays: England
ID NLM: 101710262
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
09
06
2022
revised:
29
03
2023
accepted:
05
04
2023
medline:
27
4
2023
pubmed:
27
4
2023
entrez:
27
4
2023
Statut:
epublish
Résumé
Studying the development of neural circuits in rodent models requires surgical access to the neonatal brain. Since commercially available stereotaxic and anesthetic equipment is designed for use in adults, reliable targeting of brain structures in such young animals can be challenging. Hypothermic cooling (cryoanesthesia) has been used as a preferred anesthesia approach in neonates. This commonly involves submerging neonates in ice, an approach that is poorly controllable. We have developed an affordable, simple to construct device - CryoPup - that allows for fast and robust cryoanesthesia of rodent pups. CryoPup consists of a microcontroller controlling a Peltier element and a heat exchanger. It is capable of both cooling and heating, thereby also functioning as a heating pad during recovery. Importantly, it has been designed for size compatibility with common stereotaxic frames. We validate CryoPup in neonatal mice, demonstrating that it allows for rapid, reliable and safe cryoanesthesia and subsequent recovery. This open-source device will facilitate future studies into the development of neural circuits in the postnatal brain.
Identifiants
pubmed: 37102068
doi: 10.1016/j.ohx.2023.e00417
pii: S2468-0672(23)00024-X
pmc: PMC10123246
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e00417Informations de copyright
© 2023 The Author(s).
Déclaration de conflit d'intérêts
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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