Drycells: Cell-Suspension Micro Liquid Marbles for Single-Cell Picking.
cell-picking
drycells
liquid marbles
single-cell isolation
superhydrophobic nanoparticles
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
Jul 2023
Jul 2023
Historique:
revised:
14
04
2023
received:
16
01
2023
medline:
28
7
2023
pubmed:
27
4
2023
entrez:
27
4
2023
Statut:
ppublish
Résumé
Cell-picking technology is essential for cell culturing. Although the recently developed tools enable single-cell-level picking, they rely on special skills or additional devices. In this work, a dry powder that encapsulates single to several cells with a >95% aqueous culture medium, thereby acting as a powerful cell-picking tool, is reported. The proposed "drycells" are formed by spraying a cell suspension onto a powder bed of hydrophobic fumed silica nanoparticles. The particles adsorb to the droplet surface and form a superhydrophobic shell, which prevents the drycells from coalescence. The number of encapsulated cells per drycell can be controlled by adjusting the drycell size and cell-suspension concentration. Moreover, it is possible to encapsulate a pair of normal or cancerous cells and create several cell colonies within a single drycell. A sieving process can be used to sort the drycells according to size. The size of the droplet can range from one to hundreds of micrometers. The drycells are sufficiently stiff to be collected using tweezers; however, centrifugation separates them into nanoparticles and cell-suspension layers, with the separated particles being recyclable. Various handling techniques, such as splitting coalescence and inner liquid replacement, can be used. It is believed that the application of the proposed drycells will greatly improve the accessibility and productivity of single-cell analysis.
Identifiants
pubmed: 37102709
doi: 10.1002/adma.202300486
doi:
Substances chimiques
Powders
0
Silicon Dioxide
7631-86-9
Water
059QF0KO0R
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2300486Subventions
Organisme : JSPS KAKENHI
ID : 21H01643
Organisme : JSPS KAKENHI
ID : 22K14705
Organisme : JSPS KAKENHI
ID : 20H05871
Organisme : JSPS KAKENHI
ID : 21K12696
Organisme : JSPS KAKENHI
ID : 22H00596
Informations de copyright
© 2023 Wiley-VCH GmbH.
Références
A. Khademhosseini, R. Langer, J. Borenstein, J. P. Vacanti, Proc. Natl. Acad. Sci. USA 2006, 103, 2480.
K. Uto, J. H. Tsui, C. A. DeForest, D. Kim, Prog. Polym. Sci. 2017, 65, 53.
V. Y. Kiselev, T. S. Andrews, M. Hemberg, Nat. Rev. Genet. 2019, 20, 273.
Z. Miao, P. Moreno, N. Huang, I. Papatheodorou, A. Brazma, S. A. Teichmann, Nat. Methods 2020, 17, 621.
T. Stuart, R. Satija, Nat. Rev. Genet. 2019, 20, 257.
Y. Wu, K. Zhang, Nat Rev Nephrol 2020, 16, 408.
A. O. Pisco, B. Tojo, A. McGeever, Annu Rev Biomed Data Sci 2021, 4, 207.
R. Chen, Z. Sun, D. Chen, Droplet-Based Microfluidics for Cell Encapsulation and Delivery, Elsevier Inc., Amsterdam, The Netherlands 2018.
C. J. Bustamante, Y. R. Chemla, S. Liu, M. D. Wang, Nat. Rev. Methods Prim. 2021, 1, 25.
B. P. Binks, R. Murakami, Nat. Mater. 2006, 5, 865.
K. L. Thompson, M. Williams, S. P. Armes, J. Colloid Interface Sci. 2014, 447, 217.
A. B. Subramaniam, M. Abkarian, L. Mahadevan, H. A. Stone, Langmuir 2006, 22, 10204.
K. Saleh, L. Forny, P. Guigon, I. Pezron, Chem. Eng. Res. Des. 2011, 89, 537.
P. Aussillous, D. Quéré, Nature 2001, 411, 924.
J. Saczek, X. Yao, V. Zivkovic, M. Mamlouk, D. Wang, S. S. Pramana, S. Wang, Adv. Funct. Mater. 2021, 31, 2011198.
G. McHale, M. I. Newton, Soft Matter 2011, 7, 5473.
B. Wang, K. F. Chan, F. Ji, Q. Wang, P. W. Y. Chiu, Z. Guo, L. Zhang, Adv. Sci. 2019, 6, 1802033.
M. Chen, M. P. Shah, T. B. Shelper, L. Nazareth, M. Barker, J. T. Velasquez, J. A. K. Ekberg, M. L. Vial, J. A. St John, ACS Appl. Mater. Interfaces 2019, 11, 9814.
R. K. Vadivelu, H. Kamble, A. Munaz, N. T. Nguyen, Sci. Rep. 2017, 7, 12388.
J. Aalders, L. Léger, T. Tuerlings, S. Ledda, J. van Hengel, MethodsX 2020, 7, 101065.
P. Aussillous, D. Quéré, Proc. Math. Phys. Eng. Sci. 2006, 462, 973.
N. Vogel, J. Ally, K. Bley, M. Kappl, K. Landfester, C. K. Weiss, Nanoscale 2014, 6, 6879.
J. Jin, C. H. Ooi, D. V. Dao, N. T. Nguyen, Soft Matter 2018, 14, 4160.
M. Tenjimbayashi, Y. Watanabe, Y. Nakamura, M. Naito, Adv. Mater. Interfaces 2020, 7, 2000160.
M. Tenjimbayashi, S. Samitsu, M. Naito, Adv. Funct. Mater. 2019, 29, 1900688.
Z. Yoshimitsu, A. Nakajima, T. Watanabe, K. Hashimoto, Langmuir 2002, 18, 5818.
A. Tuteja, W. Choi, J. M. Mabry, G. H. McKinley, R. E. Cohen, Proc. Natl. Acad. Sci. USA 2008, 105, 18200.
M. Tenjimbayashi, G. Hayase, T. Hiroi, T. Ueki, Adv. Mater. Interfaces 2022, 9, 2200497.
X. Deng, L. Mammen, H. J. Butt, D. Vollmer, Science 2012, 335, 67.
T. Verho, J. T. Korhonen, L. Sainiemi, V. Jokinen, C. Bower, K. Franze, S. Franssila, P. Andrew, O. Ikkala, R. H. A. Ras, Proc. Natl. Acad. Sci. USA 2012, 109, 10210.
J. Fujiwara, F. Geyer, H.-J. Butt, T. Hirai, Y. Nakamura, S. Fujii, Adv. Mater. Interfaces 2020, 7, 2001573.
A. Gallo, F. Tavares, R. Das, H. Mishra, Soft Matter 2021, 17, 7628.
N. J. Cira, A. Benusiglio, M. Prakash, Nature 2015, 519, 446.
H. M. Beakawi Al-Hashemi, O. S. Baghabra Al-Amoudi, Powder Technol. 2018, 330, 397.
M. A. Rao, Food Process Engineering: Storage and Transport of Fluid Foods in Plants, Elsevier, Amsterdam, The Netherlands 1994.
S. Ryu, P. Sen, Y. Nam, C. Lee, Phys. Rev. Lett. 2017, 118, 014501.
S. Tcholakova, N. D. Denkov, I. B. Ivanov, B. Campbell, Langmuir 2002, 18, 8960.
M. Tenjimbayashi, S. Fujii, Small 2021, 17, 2102438.
M. Tenjimbayashi, M. Naito, Adv. Funct. Mater. 2022, 32, 202204681.
B. S. Lekshmi, S. N. Varanakkottu, Langmuir 2022, 38, 11743.
K. Uto, S. S. Mano, T. Aoyagi, M. Ebara, ACS Biomater. Sci. Eng. 2016, 2, 446.
A. C. Chang, K. Uto, K. Homma, J. Nakanishi, Biomaterials 2021, 274, 120861.
X. Jia, K. Minami, K. Uto, A. C. Chang, J. P. Hill, T. Ueki, J. Nakanishi, K. Ariga, Small 2019, 15, 1804640.
X. Jia, K. Minami, K. Uto, A. C. Chang, J. P. Hill, J. Nakanishi, K. Ariga, Adv. Mater. 2020, 32, 1905942.
D. Kong, W. Megone, K. D. Q. Nguyen, S. Di Cio, M. Ramstedt, J. E. Gautrot, Nano Lett. 2018, 18, 1946.
L. Trichet, J. L.e Digabel, R. J. Hawkins, S. R. K. Vedula, M. Gupta, C. Ribrault, P. Hersen, R. Voituriez, B. Ladoux, Proc. Natl. Acad. Sci. USA 2012, 109, 6933.
J. P. Califano, C. A. Reinhart-King, Cell. Mol. Bioeng. 2010, 3, 68.
A. Mizutani, A. Kikuchi, M. Yamato, H. Kanazawa, T. Okano, Biomaterials 2008, 29, 2073.
Z. Tang, T. Okano, Regen Biomater 2014, 1, 91.
H. Cui, X. Wang, J. Wesslowski, T. Tronser, J. Rosenbauer, A. Schug, G. Davidson, A. A. Popova, P. A. Levkin, Adv. Mater. 2021, 33, 2006434.
R. Yonashiro, A. Sugiura, M. Miyachi, T. Fukuda, N. Matsushita, R. Inatome, Y. Ogata, T. Suzuki, N. Dohmae, S. Yanagi, Mol. Biol. Cell 2009, 20, 4524.
H. Hirata, O. Dobrokhotov, M. Sokabe, iScience 2020, 23, 101729.