Application of an Extracellular Matrix-Mimicking Fluorescent Polymer for the Detection of Proteolytic Venom Toxins.
SVMP
cytotoxicity
extracellular matrix
high-throughput assay
snakebite
tissue damage
toxins
Journal
Toxins
ISSN: 2072-6651
Titre abrégé: Toxins (Basel)
Pays: Switzerland
ID NLM: 101530765
Informations de publication
Date de publication:
18 04 2023
18 04 2023
Historique:
received:
23
03
2023
revised:
12
04
2023
accepted:
15
04
2023
medline:
1
5
2023
pubmed:
27
4
2023
entrez:
27
4
2023
Statut:
epublish
Résumé
The cytotoxicity caused by snake venoms is a serious medical problem that greatly contributes to the morbidity observed in snakebite patients. The cytotoxic components found in snake venoms belong to a variety of toxin classes and may cause cytotoxic effects by targeting a range of molecular structures, including cellular membranes, the extracellular matrix (ECM) and the cytoskeleton. Here, we present a high-throughput assay (384-well plate) that monitors ECM degradation by snake venom toxins via the application of fluorescent versions of model ECM substrates, specifically gelatin and collagen type I. Both crude venoms and fractionated toxins of a selection of medically relevant viperid and elapid species, separated via size-exclusion chromatography, were studied using the self-quenching, fluorescently labelled ECM-polymer substrates. The viperid venoms showed significantly higher proteolytic degradation when compared to elapid venoms, although the venoms with higher snake venom metalloproteinase content did not necessarily exhibit stronger substrate degradation than those with a lower one. Gelatin was generally more readily cleaved than collagen type I. In the viperid venoms, which were subjected to fractionation by SEC, two (
Identifiants
pubmed: 37104232
pii: toxins15040294
doi: 10.3390/toxins15040294
pmc: PMC10143632
pii:
doi:
Substances chimiques
Collagen Type I
0
Gelatin
9000-70-8
Snake Venoms
0
Toxins, Biological
0
Elapid Venoms
0
Metalloproteases
EC 3.4.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Wellcome Trust
ID : 221712/Z/20/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 221710/Z/20/Z
Pays : United Kingdom
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