Co-Selection of Bacterial Metal and Antibiotic Resistance in Soil Laboratory Microcosms.
PICT
TRFLP
antibiotic resistance
bacteria
co-selection
heavy metal resistance
soil
trace elements
Journal
Antibiotics (Basel, Switzerland)
ISSN: 2079-6382
Titre abrégé: Antibiotics (Basel)
Pays: Switzerland
ID NLM: 101637404
Informations de publication
Date de publication:
18 Apr 2023
18 Apr 2023
Historique:
received:
28
02
2023
revised:
06
04
2023
accepted:
15
04
2023
medline:
28
4
2023
pubmed:
28
4
2023
entrez:
28
4
2023
Statut:
epublish
Résumé
Accumulation of heavy metals (HMs) in agricultural soil following the application of superphosphate fertilisers seems to induce resistance of soil bacteria to HMs and appears to co-select for resistance to antibiotics (Ab). This study aimed to investigate the selection of co-resistance of soil bacteria to HMs and Ab in uncontaminated soil incubated for 6 weeks at 25 °C in laboratory microcosms spiked with ranges of concentrations of cadmium (Cd), zinc (Zn) and mercury (Hg). Co-selection of HM and Ab resistance was assessed using plate culture on media with a range of HM and Ab concentrations, and pollution-induced community tolerance (PICT) assays. Bacterial diversity was profiled via terminal restriction fragment length polymorphism (TRFLP) assay and 16S rDNA sequencing of genomic DNA isolated from selected microcosms. Based on sequence data, the microbial communities exposed to HMs were found to differ significantly compared to control microcosms with no added HM across a range of taxonomic levels.
Identifiants
pubmed: 37107134
pii: antibiotics12040772
doi: 10.3390/antibiotics12040772
pmc: PMC10135173
pii:
doi:
Types de publication
Journal Article
Langues
eng
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