A Rapid RPA-CRISPR/Cas12a Detection Method for Adulteration of Goat Milk Powder.
CRISPR/Cas12a
adulteration
food fraud
goat milk powder
rapid detection
Journal
Foods (Basel, Switzerland)
ISSN: 2304-8158
Titre abrégé: Foods
Pays: Switzerland
ID NLM: 101670569
Informations de publication
Date de publication:
07 Apr 2023
07 Apr 2023
Historique:
received:
15
02
2023
revised:
14
03
2023
accepted:
24
03
2023
medline:
28
4
2023
pubmed:
28
4
2023
entrez:
28
4
2023
Statut:
epublish
Résumé
Because of the serious adulteration of goat milk, the rapid on-site detection of goat milk powder adulteration is needed. In this study, the CRISPR/Cas12a detection system combined with recombinase polymerase amplification (RPA) was employed to qualitatively detect the adulteration of goat milk powder with cattle-derived components. Specific primers and crRNA were designed and screened. After the optimization of RPA and the Cas system, the RPA-CRISPR/Cas12a detection method was established. The detection can complete the rapid identification of cattle-derived components in 45 min, without the assistant of large equipment. The absolute detectability of the RPA-CRISPR/Cas12a assay could reach 10
Identifiants
pubmed: 37107364
pii: foods12081569
doi: 10.3390/foods12081569
pmc: PMC10137891
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : the National Scientific Foundation of China
ID : 32072316
Organisme : Generic Technique Innovation Team Construction of Modern Agriculture of Guangdong Prov-ince
ID : 2022KJ130, 2023KJ130
Organisme : Project of Guangdong Laboratory for Lingnan Modern Agriculture
ID : LNSYSZX001, P20211154-0102-02
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