New Perspectives of Multiplex Mass Spectrometry Blood Protein Quantification on Microsamples in Biological Monitoring of Elderly Patients.
DBS
LC-MS
clinical chemistry
microsampling
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
10 Apr 2023
10 Apr 2023
Historique:
received:
28
02
2023
revised:
04
04
2023
accepted:
05
04
2023
medline:
1
5
2023
pubmed:
28
4
2023
entrez:
28
4
2023
Statut:
epublish
Résumé
Blood microsampling combined with large panels of clinically relevant tests are of major interest for the development of home sampling and predictive medicine. The aim of the study was to demonstrate the practicality and medical utility of microsamples quantification using mass spectrometry (MS) in a clinical setting by comparing two types of microsamples for multiplex MS protein detection. In a clinical trial based on elderly population, we compared 2 µL of plasma to dried blood spot (DBS) with a clinical quantitative multiplex MS approach. The analysis of the microsamples allowed the quantification of 62 proteins with satisfactory analytical performances. A total of 48 proteins were significantly correlated between microsampling plasma and DBS (
Identifiants
pubmed: 37108152
pii: ijms24086989
doi: 10.3390/ijms24086989
pmc: PMC10139225
pii:
doi:
Substances chimiques
Blood Proteins
0
Types de publication
Clinical Trial
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : French National Agency for Research
ID : BuVAMASS- ANR-15-CE18-0011-01
Organisme : University Hospital Center of Montpellier
ID : UF 9114 AOI DM 2012 DBSage
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