Selecting 16S rRNA Primers for Microbiome Analysis in a Host-Microbe System: The Case of the Jellyfish
amplicon sequencing
cnidaria
jellyfish
method comparison
microbial community
next-generation sequencing
universal primers
Journal
Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893
Informations de publication
Date de publication:
06 Apr 2023
06 Apr 2023
Historique:
received:
09
03
2023
revised:
30
03
2023
accepted:
03
04
2023
medline:
28
4
2023
pubmed:
28
4
2023
entrez:
28
4
2023
Statut:
epublish
Résumé
Amplicon sequencing of the 16S rRNA gene is extensively used to characterize bacterial communities, including those living in association with eukaryotic hosts. Deciding which region of the 16S rRNA gene to analyze and selecting the appropriate PCR primers remains a major decision when initiating any new microbiome study. Based on a detailed literature survey of studies focusing on cnidarian microbiomes, we compared three commonly used primers targeting different hypervariable regions of the 16S rRNA gene, V1V2, V3V4, and V4V5, using the jellyfish
Identifiants
pubmed: 37110378
pii: microorganisms11040955
doi: 10.3390/microorganisms11040955
pmc: PMC10144005
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Israel Ministry of Science and Technology
ID : 3-15501
Organisme : Israel Ministry of Science and Technology
ID : 3-17404
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