Analytics in Extrusion-Based Bioprinting: Standardized Methods Improving Quantification and Comparability of the Performance of Bioinks.
GelMA
alginate
bioink
biomaterials
bioprinting
fibroblasts
flow cytometry
printability
Journal
Polymers
ISSN: 2073-4360
Titre abrégé: Polymers (Basel)
Pays: Switzerland
ID NLM: 101545357
Informations de publication
Date de publication:
09 Apr 2023
09 Apr 2023
Historique:
received:
28
02
2023
revised:
30
03
2023
accepted:
07
04
2023
medline:
28
4
2023
pubmed:
28
4
2023
entrez:
28
4
2023
Statut:
epublish
Résumé
Three-dimensional bioprinting and especially extrusion-based printing as a most frequently employed method in this field is constantly evolving as a discipline in regenerative medicine and tissue engineering. However, the lack of relevant standardized analytics does not yet allow an easy comparison and transfer of knowledge between laboratories regarding newly developed bioinks and printing processes. This work revolves around the establishment of a standardized method, which enables the comparability of printed structures by controlling for the extrusion rate based on the specific flow behavior of each bioink. Furthermore, printing performance was evaluated by image-processing tools to verify the printing accuracy for lines, circles, and angles. In addition, and complementary to the accuracy metrics, a dead/live staining of embedded cells was performed to investigate the effect of the process on cell viability. Two bioinks, based on alginate and gelatin methacryloyl, which differed in 1% (
Identifiants
pubmed: 37111976
pii: polym15081829
doi: 10.3390/polym15081829
pmc: PMC10144221
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Federal Ministry of Education and Research
ID : 13XP5071B
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