Analysis of Nipah Virus Replication and Host Proteome Response Patterns in Differentiated Porcine Airway Epithelial Cells Cultured at the Air-Liquid Interface.
Nipah virus
air–liquid interface culture
immune response
mass spectrometry
respiratory epithelium
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
13 04 2023
13 04 2023
Historique:
received:
13
03
2023
revised:
03
04
2023
accepted:
11
04
2023
medline:
1
5
2023
pubmed:
28
4
2023
entrez:
28
4
2023
Statut:
epublish
Résumé
Respiratory tract epithelium infection plays a primary role in Nipah virus (NiV) pathogenesis and transmission. Knowledge about infection dynamics and host responses to NiV infection in respiratory tract epithelia is scarce. Studies in non-differentiated primary respiratory tract cells or cell lines indicate insufficient interferon (IFN) responses. However, studies are lacking in the determination of complex host response patterns in differentiated respiratory tract epithelia for the understanding of NiV replication and spread in swine. Here we characterized infection and spread of NiV in differentiated primary porcine bronchial epithelial cells (PBEC) cultivated at the air-liquid interface (ALI). After the initial infection of only a few apical cells, lateral spread for 12 days with epithelium disruption was observed without releasing substantial amounts of infectious virus from the apical or basal sides. Deep time course proteomics revealed pronounced upregulation of genes related to type I/II IFN, immunoproteasomal subunits, transporter associated with antigen processing (TAP)-mediated peptide transport, and major histocompatibility complex (MHC) I antigen presentation. Spliceosomal factors were downregulated. We propose a model in which NiV replication in PBEC is slowed by a potent and broad type I/II IFN host response with conversion from 26S proteasomes to immunoproteasomal antigen processing and improved MHC I presentation for adaptive immunity priming. NiV induced cytopathic effects could reflect the focal release of cell-associated NiV, which may contribute to efficient airborne viral spread between pigs.
Identifiants
pubmed: 37112941
pii: v15040961
doi: 10.3390/v15040961
pmc: PMC10143807
pii:
doi:
Substances chimiques
Proteome
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
Immunity. 2004 Aug;21(2):155-65
pubmed: 15308097
EMBO J. 2021 Mar 1;40(5):e105912
pubmed: 33283287
J Virol. 2013 Mar;87(6):3284-94
pubmed: 23302882
Nat Genet. 2000 May;25(1):25-9
pubmed: 10802651
J Virol. 2017 Oct 13;91(21):
pubmed: 28835499
Physiol Rep. 2021 Feb;9(4):e14761
pubmed: 33625796
J Virol. 2018 Feb 26;92(6):
pubmed: 29321315
J Infect Dis. 2020 May 11;221(Suppl 4):S395-S400
pubmed: 31665348
Nat Methods. 2016 Sep;13(9):731-40
pubmed: 27348712
Curr Opin Virol. 2011 Dec;1(6):519-25
pubmed: 22328912
PLoS Pathog. 2010 Nov 11;6(11):e1001186
pubmed: 21085610
Immunity. 1996 Apr;4(4):357-66
pubmed: 8612130
Respir Res. 2020 May 12;21(1):111
pubmed: 32398133
PLoS Negl Trop Dis. 2018 Mar 14;12(3):e0006343
pubmed: 29538374
Bioinformatics. 2009 Apr 15;25(8):1091-3
pubmed: 19237447
J Clin Invest. 2006 Nov;116(11):3006-14
pubmed: 17039255
F1000Res. 2019 Oct 16;8:
pubmed: 31656582
J Biochem. 1994 Feb;115(2):257-69
pubmed: 8206875
J Virol. 2000 Nov;74(21):9972-9
pubmed: 11024125
J Virol. 2014 Nov;88(22):13099-110
pubmed: 25210190
PLoS Pathog. 2021 Aug 12;17(8):e1009458
pubmed: 34383863
Emerg Infect Dis. 2006 Feb;12(2):235-40
pubmed: 16494748
J Virol. 2009 Aug;83(16):7828-41
pubmed: 19515782
Electrophoresis. 1988 Jun;9(6):255-62
pubmed: 2466658
Virology. 2010 Aug 15;404(1):78-88
pubmed: 20552729
PLoS One. 2014 May 13;9(5):e97233
pubmed: 24823948
Nat Biotechnol. 2008 Dec;26(12):1367-72
pubmed: 19029910
J Gen Virol. 2020 Jan;101(1):44-58
pubmed: 31793855
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Cells. 2020 Oct 30;9(11):
pubmed: 33143316
PLoS One. 2015 Aug 19;10(8):e0135675
pubmed: 26287616
Indian J Med Res. 2006 Apr;123(4):553-60
pubmed: 16783047
J Virol. 2006 Aug;80(16):7929-38
pubmed: 16873250
Rev Med Virol. 2019 Jan;29(1):e2010
pubmed: 30251294
Am J Physiol Lung Cell Mol Physiol. 2004 Aug;287(2):L318-31
pubmed: 15246982
Mol Cell. 2020 Dec 17;80(6):1104-1122.e9
pubmed: 33259812
Genome Res. 2003 Nov;13(11):2498-504
pubmed: 14597658
J Gen Virol. 2016 May;97(5):1077-1086
pubmed: 26932515
Nucleic Acids Res. 2021 Jan 8;49(D1):D545-D551
pubmed: 33125081
Emerg Infect Dis. 2005 Oct;11(10):1594-7
pubmed: 16318702
Mol Cell Proteomics. 2020 Jun;19(6):1058-1069
pubmed: 32156793
Emerg Infect Dis. 2001 May-Jun;7(3):439-41
pubmed: 11384522
PLoS Pathog. 2016 Sep 13;12(9):e1005880
pubmed: 27622505
Nucleic Acids Res. 2019 Jan 8;47(D1):D442-D450
pubmed: 30395289
J Virol. 2004 May;78(10):5358-67
pubmed: 15113915
Sci Rep. 2015 May 19;5:10230
pubmed: 25989070
PLoS One. 2008 Sep 19;3(9):e3247
pubmed: 18802471
J Proteome Res. 2022 Feb 4;21(2):459-469
pubmed: 34982558
Microbes Infect. 2001 Apr;3(4):315-22
pubmed: 11334749
Nat Commun. 2021 Dec 7;12(1):7092
pubmed: 34876592
Clin Infect Dis. 2008 Apr 1;46(7):977-84
pubmed: 18444812
Lancet. 1999 Oct 9;354(9186):1257-9
pubmed: 10520635
Nat Methods. 2009 May;6(5):359-62
pubmed: 19377485
Ann Neurol. 1999 Sep;46(3):428-32
pubmed: 10482278
FEBS Lett. 2016 Aug;590(15):2494-511
pubmed: 27350027
J Virol. 2013 Mar;87(5):2974-8
pubmed: 23269789
Nucleic Acids Res. 2000 Jan 1;28(1):27-30
pubmed: 10592173
Nat Commun. 2018 Aug 3;9(1):3057
pubmed: 30076298
PLoS Negl Trop Dis. 2017 Apr 7;11(4):e0005532
pubmed: 28388650
Methods Mol Med. 2005;107:183-206
pubmed: 15492373
J Gen Virol. 2011 Sep;92(Pt 9):2093-2104
pubmed: 21593271
Front Immunol. 2018 Mar 28;9:635
pubmed: 29643856
Nucleic Acids Res. 2017 Jan 4;45(D1):D635-D642
pubmed: 27899575