Isolation and Differentiation of Neurons and Glial Cells from Olfactory Epithelium in Living Subjects.
Glia
Neurons
Neuropsychiatric diseases
Neurospheres
Olfactory epithelium
PSA-NCAM
Journal
Molecular neurobiology
ISSN: 1559-1182
Titre abrégé: Mol Neurobiol
Pays: United States
ID NLM: 8900963
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
17
01
2023
accepted:
19
04
2023
medline:
28
6
2023
pubmed:
29
4
2023
entrez:
28
4
2023
Statut:
ppublish
Résumé
The study of psychiatric and neurological diseases requires the substrate in which the disorders occur, that is, the nervous tissue. Currently, several types of human bio-specimens are being used for research, including postmortem brains, cerebrospinal fluid, induced pluripotent stem (iPS) cells, and induced neuronal (iN) cells. However, these samples are far from providing a useful predictive, diagnostic, or prognostic biomarker. The olfactory epithelium is a region close to the brain that has received increased interest as a research tool for the study of brain mechanisms in complex neuropsychiatric and neurological diseases. The olfactory sensory neurons are replaced by neurogenesis throughout adult life from stem cells on the basement membrane. These stem cells are multipotent and can be propagated in neurospheres, proliferated in vitro and differentiated into multiple cell types including neurons and glia. For all these reasons, olfactory epithelium provides a unique resource for investigating neuronal molecular markers of neuropsychiatric and neurological diseases. Here, we describe the isolation and culture of human differentiated neurons and glial cells from olfactory epithelium of living subjects by an easy and non-invasive exfoliation method that may serve as a useful tool for the research in brain diseases.
Identifiants
pubmed: 37118325
doi: 10.1007/s12035-023-03363-2
pii: 10.1007/s12035-023-03363-2
pmc: PMC10293402
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4472-4487Subventions
Organisme : Ministerio de Ciencia e Innovación
ID : PID2019-106404RB-I00
Organisme : Plan Nacional sobre Drogas
ID : PNSD 2019I021
Organisme : Eusko Jaurlaritza
ID : 2019111082
Organisme : Eusko Jaurlaritza
ID : IT1211/19
Organisme : Eusko Jaurlaritza
ID : IT1512/22
Informations de copyright
© 2023. The Author(s).
Références
Cell. 1990 Feb 23;60(4):585-95
pubmed: 1689217
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
J Neurosci Res. 2002 Nov 1;70(3):327-34
pubmed: 12391592
Int J Mol Sci. 2021 Feb 09;22(4):
pubmed: 33572157
Annu Rev Neurosci. 1995;18:159-92
pubmed: 7605059
Transl Psychiatry. 2015 Oct 20;5:e663
pubmed: 26485547
Dis Model Mech. 2010 Nov-Dec;3(11-12):785-98
pubmed: 20699480
Stem Cell Res. 2015 Nov;15(3):731-741
pubmed: 26613348
Circ Res. 2003 Apr 4;92(6):598-608
pubmed: 12676811
Eur J Neurosci. 1991;3(3):209-215
pubmed: 12106197
Neuropsychopharmacology. 2014 Jan;39(1):65-87
pubmed: 24091486
Int J Neuropsychopharmacol. 2021 Oct 23;24(10):759-775
pubmed: 34216465
Front Neurosci. 2015 Oct 08;9:367
pubmed: 26500487
Cells. 2021 Jul 04;10(7):
pubmed: 34359860
J Neurosci Methods. 2011 Sep 30;201(1):35-45
pubmed: 21787803
Histochem Cell Biol. 1998 Mar;109(3):231-9
pubmed: 9541471
Curr Opin Psychiatry. 2017 May;30(3):176-183
pubmed: 28333692
Biotech Histochem. 2005 Sep-Dec;80(5-6):189-200
pubmed: 16720519
Schizophr Res. 2006 Feb 28;82(2-3):163-73
pubmed: 16406496
Adv Exp Med Biol. 2010;671:13-22
pubmed: 20455492
Sci Rep. 2016 Jul 18;6:30069
pubmed: 27425640
Sci Rep. 2020 Nov 12;10(1):19694
pubmed: 33184395
Nat Neurosci. 2020 Mar;23(3):323-326
pubmed: 32066986
Exp Cell Res. 2005 Jun 10;306(2):349-56
pubmed: 15925591
J Biol Chem. 1986 Mar 5;261(7):3396-401
pubmed: 3512556
Rev Neurosci. 2001;12(4):297-310
pubmed: 11783716
Methods Mol Biol. 2013;1059:107-14
pubmed: 23934838
Prog Neurobiol. 2012 Jul;98(1):1-15
pubmed: 22609484
Exp Neurol. 2011 Jun;229(2):308-23
pubmed: 21376038
Transl Psychiatry. 2015 Mar 17;5:e527
pubmed: 25781226
Transl Psychiatry. 2018 Apr 18;8(1):81
pubmed: 29666369
Proc Natl Acad Sci U S A. 1986 Jun;83(12):4327-31
pubmed: 3459176
Anat Rec (Hoboken). 2013 Sep;296(9):1435-52
pubmed: 23904071
Mol Neurobiol. 2021 Apr;58(4):1695-1710
pubmed: 33237429
Mol Neurobiol. 2018 Mar;55(3):2516-2523
pubmed: 28391555
Eur Psychiatry. 2019 Sep;61:23-32
pubmed: 31260908
Front Mol Neurosci. 2022 Sep 16;15:974890
pubmed: 36187353
Schizophr Bull. 2020 Dec 1;46(6):1547-1557
pubmed: 32249318
J Pers Med. 2022 Aug 20;12(8):
pubmed: 36013289
J Neurosci Methods. 2019 Jan 15;312:126-138
pubmed: 30472070
Mol Neurobiol. 2016 Apr;53(3):1637-1647
pubmed: 25680637
Mol Biol Rep. 2021 Apr;48(4):3649-3663
pubmed: 33765252
Dev Dyn. 2005 Jun;233(2):496-515
pubmed: 15782416
Anat Rec. 1999 Aug 15;257(4):137-48
pubmed: 10467245
Neurosci Biobehav Rev. 2015 Aug;55:612-26
pubmed: 26092265
Nat Neurosci. 2010 Oct;13(10):1161-9
pubmed: 20877280