Controversy and Consensus on Indications for Sperm DNA Fragmentation Testing in Male Infertility: A Global Survey, Current Guidelines, and Expert Recommendations.

DNA fragmentation Delphi method Male infertility Practice guidelines Sperm Survey

Journal

The world journal of men's health
ISSN: 2287-4208
Titre abrégé: World J Mens Health
Pays: Korea (South)
ID NLM: 101596899

Informations de publication

Date de publication:
07 2023
Historique:
received: 23 12 2022
accepted: 02 01 2023
medline: 29 4 2023
pubmed: 29 4 2023
entrez: 29 4 2023
Statut: ppublish

Résumé

Sperm DNA fragmentation (SDF) testing was recently added to the sixth edition of the World Health Organization laboratory manual for the examination and processing of human semen. Many conditions and risk factors have been associated with elevated SDF; therefore, it is important to identify the population of infertile men who might benefit from this test. The purpose of this study was to investigate global practices related to indications for SDF testing, compare the relevant professional society guideline recommendations, and provide expert recommendations. Clinicians managing male infertility were invited to take part in a global online survey on SDF clinical practices. This was conducted following the CHERRIES checklist criteria. The responses were compared to professional society guideline recommendations related to SDF and the appropriate available evidence. Expert recommendations on indications for SDF testing were then formulated, and the Delphi method was used to reach consensus. The survey was completed by 436 experts from 55 countries. Almost 75% of respondents test for SDF in all or some men with unexplained or idiopathic infertility, 39% order it routinely in the work-up of recurrent pregnancy loss (RPL), and 62.2% investigate SDF in smokers. While 47% of reproductive urologists test SDF to support the decision for varicocele repair surgery when conventional semen parameters are normal, significantly fewer general urologists (23%; p=0.008) do the same. Nearly 70% would assess SDF before assisted reproductive technologies (ART), either always or for certain conditions. Recurrent ART failure is a common indication for SDF testing. Very few society recommendations were found regarding SDF testing. This article presents the largest global survey on the indications for SDF testing in infertile men, and demonstrates diverse practices. Furthermore, it highlights the paucity of professional society guideline recommendations. Expert recommendations are proposed to help guide clinicians.

Identifiants

pubmed: 37118960
pii: 41.e43
doi: 10.5534/wjmh.220282
pmc: PMC10307662
doi:

Types de publication

Journal Article

Langues

eng

Pagination

575-602

Informations de copyright

Copyright © 2023 Korean Society for Sexual Medicine and Andrology.

Déclaration de conflit d'intérêts

The authors have nothing to disclose.

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Auteurs

Ashok Agarwal (A)

Global Andrology Forum, American Center for Reproductive Medicine, Moreland Hills, OH, USA.
Cleveland Clinic Foundation, Cleveland, OH, USA. agarwaa32099@outlook.com.

Ala'a Farkouh (A)

Global Andrology Forum, American Center for Reproductive Medicine, Moreland Hills, OH, USA.

Ramadan Saleh (R)

Department of Dermatology, Venereology and Andrology, Faculty of Medicine, Sohag University, Sohag, Egypt.

Taha Abo-Almagd Abdel-Meguid Hamoda (TA)

Department of Urology, King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Urology, Faculty of Medicine, Minia University, Minia, Egypt.

Ahmed M Harraz (AM)

Urology and Nephrology Center, Mansoura University, Mansoura, Egypt.
Department of Surgery, Urology Unit, Farwaniya Hospital, Farwaniya, Kuwait.
Department of Urology, Sabah Al Ahmad Urology Center, Kuwait City, Kuwait.

Parviz Kavoussi (P)

Department of Reproductive Urology, Austin Fertility & Reproductive Medicine/Westlake IVF, Austin, TX, USA.

Mohamed Arafa (M)

Department of Urology, Hamad Medical Corporation, Doha, Qatar.
Department of Urology, Weill Cornell Medical-Qatar, Doha, Qatar.
Department of Andrology, Sexology and STIs, Faculty of Medicine, Cairo University, Cairo, Egypt.

Gianmaria Salvio (G)

Department of Endocrinology, Polytechnic University of Marche, Ancona, Italy.

Amarnath Rambhatla (A)

Department of Urology, Henry Ford Health System, Vattikuti Urology Institute, Detroit, MI, USA.

Tuncay Toprak (T)

Department of Urology, Fatih Sultan Mehmet Training and Research Hospital, University of Health Sciences, Istanbul, Turkey.

Murat Gül (M)

Department of Urology, Selçuk University School of Medicine, Konya, Turkey.

Nguyen Ho Vinh Phuoc (NHV)

Department of Andrology, Binh Dan Hospital, Ho Chi Minh City, Vietnam.
Department of Urology and Andrology, Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam.

Florence Boitrelle (F)

Reproductive Biology, Fertility Preservation, Andrology, CECOS, Poissy Hospital, Poissy, France.
Department of Biology, Reproduction, Epigenetics, Environment and Development, Paris Saclay University, UVSQ, INRAE, BREED, Jouy-en-Josas, France.

Ponco Birowo (P)

Department of Urology, Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

Ramy Abou Ghayda (RA)

Urology Institute, University Hospitals, Case Western Reserve University, Cleveland, OH, USA.

Rossella Cannarella (R)

Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.

Shinnosuke Kuroda (S)

Department of Urology, Cleveland Clinic, Cleveland, OH, USA.

Damayanthi Durairajanayagam (D)

Department of Physiology, Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Selangor, Malaysia.

Armand Zini (A)

Department of Surgery, McGill University, Montreal, QC, Canada.

Christine Wyns (C)

Department of Gynaecology-Andrology, Cliniques universitaires Saint-Luc, Université Catholique de Louvain, Brussels, Belgium.

Selcuk Sarikaya (S)

Department of Urology, Gülhane Research and Training Hospital, University of Health Sciences, Ankara, Turkey.

Kelton Tremellen (K)

Department of Obstetrics Gynaecology and Reproductive Medicine, Flinders University, Bedford Park, Australia.

Taymour Mostafa (T)

Department of Andrology, Sexology and STIs, Faculty of Medicine, Cairo University, Cairo, Egypt.

Ioannis Sokolakis (I)

Department of Urology, Martha-Maria Hospital Nuremberg, Nuremberg, Germany.

Donald P Evenson (DP)

SCSA Diagnostics, Brookings, SD, USA.

Ralf Henkel (R)

Department of Metabolism, Digestion and Reproduction, Imperial College London, London, UK.
Department of Medical Bioscience, University of the Western Cape, Bellville, South Africa.
LogixX Pharma, Theale, Berkshire, UK.

Wael Zohdy (W)

Department of Andrology, Sexology and STIs, Faculty of Medicine, Cairo University, Cairo, Egypt.

Eric Chung (E)

Department of Urology, Princess Alexandra Hospital, University of Queensland, Brisbane, QLD, Australia.

Imad Ziouziou (I)

Department of Urology, College of Medicine and Pharmacy, Ibn Zohr University, Agadir, Morocco.

Marco Falcone (M)

Department of Urology, Molinette Hospital, A.O.U. Città della Salute e della Scienza, University of Turin, Torino, Italy.

Giorgio I Russo (GI)

Urology Section, University of Catania, Catania, Italy.

Manaf Al-Hashimi (M)

Department of Urology, Burjeel Hospital, Abu Dhabi, UAE.
Department of Urology, Clinical Urology, Khalifa University, College of Medicine and Health Sciences, Abu Dhabi, UAE.

Aldo E Calogero (AE)

Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.

Edmund Ko (E)

Department of Urology, Loma Linda University Health, Loma Linda, CA, USA.

Giovanni Colpi (G)

Andrology and IVF Center, Next Fertility Procrea, Lugano, Switzerland.

Sheena Lewis (S)

Examen Lab Ltd., Northern Ireland, UK.

Ege Can Serefoglu (EC)

Department of Urology, Biruni University School of Medicine, Istanbul, Turkey.

Fahmi Bahar (F)

Andrology Section, Siloam Sriwijaya Hospital, Palembang, Indonesia.

Marlon Martinez (M)

Section of Urology, Department of Surgery, University of Santo Tomas Hospital, Manila, Philippines.

Quang Nguyen (Q)

Center for Andrology and Sexual Medicine, Viet Duc University Hospital, Hanoi, Vietnam.
Department of Urology, Andrology and Sexual Medicine, University of Medicine and Pharmacy, Vietnam National University, Hanoi, Vietnam.

Rafael F Ambar (RF)

Department of Urology, Centro Universitario em Saude do ABC, Santo André, Brazil.
Andrology Group at Ideia Fertil Institute of Human Reproduction, Santo André, Brazil.

Mustafa Emre Bakircioglu (ME)

SENSART Clinic, Urology, Fulya, Istanbul, Turkiye.

Hussein Kandil (H)

Fakih IVF Fertility Center, Abu Dhabi, UAE.

Nasser Mogharabian (N)

Sexual Health and Fertility Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.

Marjan Sabbaghian (M)

Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Hisanori Taniguchi (H)

Department of Urology and Andrology, Kansai Medical University, Hirakata, Osaka, Japan.

Akira Tsujimura (A)

Department of Urology, Juntendo University Urayasu Hospital, Chiba, Japan.

Hesamoddin Sajadi (H)

Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Wael Ibrahim (W)

Department of Obstetrics Gynaecology and Reproductive Medicine, Fertility Care Center in Cairo, Cairo, Egypt.

Widi Atmoko (W)

Department of Urology, Cipto Mangunkusumo General Hospital, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.

Paraskevi Vogiatzi (P)

Andromed Health & Reproduction, Fertility & Reproductive Health Diagnostic Center, Athens, Greece.

Sezgin Gunes (S)

Department of Medical Biology, Medical Faculty, Ondokuz Mayis University, Samsun, Turkey.

Mohammad Ali Sadighi Gilani (MA)

Department of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

Shubhadeep Roychoudhury (S)

Department of Life Science and Bioinformatics, Assam University, Silchar, India.

Nur Dokuzeylül Güngör (ND)

Department of Obstetrics and Gynecology, Reproductive Endocrinology and IVF Unit, School of Medicine, Bahcesehir University, Istanbul, Turkey.

Lukman Hakim (L)

Department of Urology, Faculty of Medicine Universitas Airlangga, Universitas Airlangga Teaching Hospital, Surabaya, Indonesia.

Ricky Adriansjah (R)

Department of Urology, Faculty of Medicine Universitas Padjajaran, Hasan Sadikin General Hospital, Bandung, Indonesia.

Priyank Kothari (P)

Department of Urology, Topiwala National Medical College, B.Y.L Nair Ch Hospital, Mumbai, India.

Sunil Jindal (S)

Department of Andrology and Reproductive Medicine, Jindal Hospital, Meerut, India.

Edouard Amar (E)

Department of Urology, American Hospital of Paris, Paris, France.

Hyun Jun Park (HJ)

Department of Urology, Pusan National University School of Medicine, Busan, Korea.
Medical Research Institute of Pusan National University Hospital, Busan, Korea.

Tran Quang Tien Long (TQT)

Department of Obstetrics and Gynecology, Hanoi Obstetric and Gynecology Hospital, Hanoi, Vietnam.

Sheryl Homa (S)

Department of Biosciences, University of Kent, Canterbury, UK.

Vilvapathy Senguttuvan Karthikeyan (VS)

Andrology Unit, Department of Urology, Apollo Hospitals, Greams Road, Chennai, India.

Birute Zilaitiene (B)

Institute of Endocrinology, Medical Academy, Lithuanian University of Health Sciences, Kaunas, Lithuania.

Israel Maldonado Rosas (I)

IVF Laboratory, CITMER Reproductive Medicine, Mexico City, Mexico.

Angelo Marino (A)

Reproductive Medicine Unit, ANDROS Day Surgery Clinic, Palermo, Italy.

Edoardo Pescatori (E)

Andrology and Reproductive Medicine Unit, Gynepro Medical, Bologna, Italy.

Cevahir Ozer (C)

Department of Urology, Faculty of Medicine, Baskent University, Ankara, Turkey.

Hamed Akhavizadegan (H)

Department of Urology, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran.

Nicolas Garrido (N)

IVI Foundation, Instituto de Investigación Sanitaria La Fe (IIS La Fe), Valencia, Spain.

Gian Maria Busetto (GM)

Department of Urology and Renal Transplantation, University of Foggia, Policlinico Riuniti, Foggia, Italy.

Aram Adamyan (A)

IVF Department, Astghik Medical Center, Yerevan, Armenia.

Mohamed Al-Marhoon (M)

Division of Urology, Department of Surgery, Sultan Qaboos University, Muscat, Sultanate of Oman.

Haitham Elbardisi (H)

Department of Urology, Hamad Medical Corporation, Doha, Qatar.
Department of Urology, Weill Cornell Medical-Qatar, Doha, Qatar.

Parisa Dolati (P)

Department of Animal Science, Faculty of Agriculture, University of Shiraz, Iran.

Mahsa Darbandi (M)

Fetal Health Research Center, Hope Generation Foundation, Tehran, Iran.
Gene Therapy and Regenerative Medicine Research Center, Hope Generation Foundation, Tehran, Iran.

Sara Darbandi (S)

Fetal Health Research Center, Hope Generation Foundation, Tehran, Iran.
Gene Therapy and Regenerative Medicine Research Center, Hope Generation Foundation, Tehran, Iran.

Giancarlo Balercia (G)

Department of Endocrinology, Polytechnic University of Marche, Ancona, Italy.

Germar-Michael Pinggera (GM)

Department of Urology, Innsbruck Medical University, Innsbruck, Austria.

Sava Micic (S)

Department of Andrology, Uromedica Polyclinic, Belgrade, Serbia.

Christopher Chee Kong Ho (CCK)

Department of Surgery, School of Medicine, Taylor's University, Subang Jaya, Selangor, Malaysia.

Mohamad Moussa (M)

Department of Urology, Lebanese University, Beirut, Lebanon.
Department of Urology, Al Zahraa Hospital, UMC, Lebanon.

Mirko Preto (M)

Department of Urology, Molinette Hospital, A.O.U. Città della Salute e della Scienza, University of Turin, Torino, Italy.

Cătălina Zenoaga-Barbăroșie (C)

Department of Genetics, Faculty of Biology, University of Bucharest, Bucharest, Romania.

Ryan P Smith (RP)

Department of Urology, University of Virginia School of Medicine, Virginia, USA.

Raghavender Kosgi (R)

Department of Urology, Andrology and Renal Transplant, AIG Hospitals, Hyderabad, India.

Jean de la Rosette (J)

Department of Urology, Istanbul Medipol Mega University Hospital, Istanbul, Turkey.

Ahmed I El-Sakka (AI)

Department of Urology, Suez Canal University, Ismailia, Egypt.

Saad Mohammed Abumelha (SM)

Division of Urology, Department of Surgery, King Abdulaziz Medical City, Riyadh, Saudi Arabia.

Tiago Cesar Mierzwa (TC)

Androlab, Fertility Clinic, Curitiba, PR, Brazil.

Teng Aik Ong (TA)

Department of Surgery, University of Malaya, Kuala Lumpur, Malaysia.

Saleem A Banihani (SA)

Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.

Kasonde Bowa (K)

Department of Urology, University of Lusaka, Lusaka, Zambia.

Shinichiro Fukuhara (S)

Department of Urology, Graduate School of Medicine, Osaka University, Osaka, Japan.

Luca Boeri (L)

Department of Urology, IRCCS Fondazione Ca' Granda, Ospedale Maggiore Policlinico, Milan, Italy.

Yavuz Onur Danacıoğlu (YO)

Department of Urology, University of Health Science Istanbul Bakırköy Dr. Sadi Konuk Training and Research Hospital, Istanbul, Turkey.

Fatih Gokalp (F)

Department of Urology, Hatay Mustafa Kemal University, Antakya, Turkey.

Osama Mohamed Selim (OM)

Department of Andrology, Sexology and STIs, Faculty of Medicine, Cairo University, Cairo, Egypt.

Chak-Lam Cho (CL)

S. H. Ho Urology Centre, Department of Surgery, The Chinese University of Hong Kong, Hong Kong.

Nicholas N Tadros (NN)

Division of Urology, Southern Illinois University, Springfield, IL, USA.

Muhammet Rasit Ugur (MR)

IVF Michigan, Bloomfield Hills, MI, USA.

Mehmet Serkan Ozkent (MS)

Department of Urology, Konya City Hospital, Konya, Turkey.

Peter Chiu (P)

S. H. Ho Urology Centre, Department of Surgery, The Chinese University of Hong Kong, Hong Kong.

Arif Kalkanli (A)

Department of Urology, Taksim Education and Research Hospital, Istanbul, Turkey.

Kareim Khalafalla (K)

Department of Urology, Hamad Medical Corporation, Doha, Qatar.
Department of Urology, University of Illinois, Chicago, IL, USA.

Ranjit B Vishwakarma (RB)

Department of Urology, Lilavati Hospital and Research Centre, Mumbai, India.

Federica Finocchi (F)

Department of Endocrinology, Polytechnic University of Marche, Ancona, Italy.
Unit of Andrology and Reproductive Medicine, University of Padua, Padua, Italy.

Sotiris Andreadakis (S)

Private Practice, Thessaloniki, Greece.

Carlo Giulioni (C)

Department of Urology, Polytechnic University of Marche Region, Ancona, Italy.

Gökhan Çeker (G)

Department of Urology, Başakşehir Çam and Sakura City Hospital, Istanbul, Turkey.
Department of Embryology and Histology, Zonguldak Bülent Ecevit University Institute of Health Sciences, Zonguldak, Turkey.

Erman Ceyhan (E)

Department of Urology, Faculty of Medicine, Baskent University, Ankara, Turkey.

Vineet Malhotra (V)

Department of Urology, SCM Clinic and Hospital, New Delhi, India.

Mehmet Yilmaz (M)

Department of Urology, Faculty of Medicine, University of Freiburg-Medical Centre, Freiburg, Germany.

Massimiliano Timpano (M)

Department of Urology, Molinette Hospital, A.O.U. Città della Salute e della Scienza, University of Turin, Torino, Italy.

Trenton L Barrett (TL)

Perth Urology Clinic, Perth, WA, Australia.

Shannon Hee Kyung Kim (SHK)

IVF Australia, Sydney, Australia.
Macquarie School of Medicine, Macquaire University, Sydney, Australia.

Sun-Tae Ahn (ST)

Department of Urology, Korea University College of Medicine, Seoul, Korea.

Filippo Giacone (F)

Centro HERA, Unità di Medicina della Riproduzione, Sant'Agata Li Battiati, Catania, Italy.

Ayad Palani (A)

Research Centre, University of Garmian, Kalar, Iraq.

Gede Wirya Kusuma Duarsa (GWK)

Department of Urology, Udayana University, Denpasar, Indonesia.

Ates Kadioglu (A)

Section of Andrology, Department of Urology, Istanbul University, Istanbul, Turkey.

Franco Gadda (F)

Department of Urology, IRCCS Fondazione Ca' Granda, Ospedale Maggiore Policlinico, Milan, Italy.

Daniel Suslik Zylbersztejn (DS)

Fleury Group and Hospital Israelita Albert Einstein, São Paulo, Brazil.

Kaan Aydos (K)

Department of Urology, Ankara University, Ankara, Turkey.

Deniz Kulaksız (D)

Department of Obstetrics and Gynecology, University of Health Sciences Kanuni Training and Research Hospital, Trabzon, Turkey.

Deepak Gupte (D)

Department of Urology, Bombay Hospital and Medical Research Center, Mumbai, India.

Gokhan Calik (G)

Department of Urology, Istanbul Medipol University, Istanbul, Turkey.

Keshab Kumar Karna (KK)

Department of Molecular Cell and Cancer Biology, University of Massachusetts Medical School, Worcester, MA, USA.

Panagiotis Drakopoulos (P)

Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussels, Belgium.
IVF Athens, Athens, Greece.

Aykut Baser (A)

Department of Urology, Bandirma Onyedi Eylül University, Balikesir, Turkey.

Vijay Kumar (V)

Department of Microbiology, Kurukshetra University, Kurukshetra, India.

Juan Manuel Corral Molina (JMC)

Urology Department, Hospital Clinic of Barcelona, Barcelona, Spain.

Osvaldo Rajmil (O)

Department of Andrology, Fundacio Puigvert, Barcelona, Spain.

Raphael H Ferreira (RH)

Department of Urology, Santa Casa de Ribeirão Preto, São Paulo, Brazil.

Sofia Leonardi (S)

Central Laboratory, Hospital Publico Materno Infantil de Salta, Salta, Argentina.

Armen Avoyan (A)

Urology Department, Astghik Medical Center, Yerevan, Armenia.

Emrullah Sogutdelen (E)

Department of Urology, Bolu Abant Izzet Baysal University, Bolu, Turkey.

Giorgio Franco (G)

Department of Urology, Policlinico Umberto I, Sapienza University of Rome, Rome, Italy.

Jonathan Ramsay (J)

Department of Andrology, Hammersmith Hospital, London, UK.

Liliana Ramirez (L)

IVF Laboratory, CITMER Reproductive Medicine, Mexico City, Mexico.

Rupin Shah (R)

Department of Urology, Lilavati Hospital and Research Centre, Mumbai, India.

Classifications MeSH