Amplicon Sequencing Reveals Complex Infection in Infants Congenitally Infected With Trypanosoma Cruzi and Informs the Dynamics of Parasite Transmission.
Trypanosoma cruzi
amplicon sequencing
congenital chagas
Journal
The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675
Informations de publication
Date de publication:
15 09 2023
15 09 2023
Historique:
received:
24
11
2022
accepted:
26
04
2023
pmc-release:
29
04
2024
medline:
18
9
2023
pubmed:
29
4
2023
entrez:
29
4
2023
Statut:
ppublish
Résumé
Congenital transmission of Trypanosoma cruzi is an important source of new Chagas infections worldwide. The mechanisms of congenital transmission remain poorly understood, but there is evidence that parasite factors are involved. Investigating changes in parasite strain diversity during transmission could provide insight into the parasite factors that influence the process. Here we use amplicon sequencing of a single copy T. cruzi gene to evaluate the diversity of infection in clinical samples from Chagas positive mothers and their infected infants. Several infants and mothers were infected with multiple parasite strains, mostly of the same TcV lineage, and parasite strain diversity was higher in infants than mothers. Two parasite haplotypes were detected exclusively in infant samples, while one haplotype was never found in infants. Together, these data suggest multiple parasites initiate a congenital infection and that parasite factors influence the probability of vertical transmission.
Identifiants
pubmed: 37119236
pii: 7147086
doi: 10.1093/infdis/jiad125
pmc: PMC10503952
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
769-776Subventions
Organisme : NIAID NIH HHS
ID : R01 AI151295
Pays : United States
Organisme : NIAID NIH HHS
ID : K24 AI134990
Pays : United States
Organisme : NIH HHS
ID : R01AI151295, K23AI113197, R01AI136722
Pays : United States
Organisme : NCATS NIH HHS
ID : TL1 TR002491
Pays : United States
Organisme : NIAID NIH HHS
ID : K23 AI113197
Pays : United States
Organisme : FIC NIH HHS
ID : D43 TW010074
Pays : United States
Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of Infectious Diseases Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Déclaration de conflit d'intérêts
Potential conflicts of interest. All authors: No reported conflicts of interest.
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