In situ assessment of Mindin as a biomarker of podocyte lesions in diabetic nephropathy.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2023
2023
Historique:
received:
19
11
2022
accepted:
07
04
2023
medline:
4
5
2023
pubmed:
2
5
2023
entrez:
2
5
2023
Statut:
epublish
Résumé
Diabetic nephropathy (DN) is the leading cause of chronic kidney disease and end-stage renal failure worldwide. Several mechanisms are involved in the pathogenesis of this disease, which culminate in morphological changes such as podocyte injury. Despite the complex diagnosis and pathogenesis, limited attempts have been made to establish new biomarkers for DN. The higher concentration of Mindin protein in the urine of patients with type 2 diabetes mellitus suggests that it plays a role in DN. Therefore, this study investigated whether in situ protein expression of Mindin can be considered a potential DN biomarker. Fifty renal biopsies from patients diagnosed with DN, 57 with nondiabetic glomerular diseases, including 17 with focal segmental glomerulosclerosis (FSGS), 14 with minimal lesion disease (MLD) and 27 with immunoglobulin A nephropathy (IgAN), and 23 adult kidney samples from autopsies (control group) were evaluated for Mindin expression by immunohistochemistry. Podocyte density was inferred by Wilms' tumor 1 (WT1) immunostaining, while foot process effacement was assessed by transmission electron microscopy. Receiver operative characteristic (ROC) analysis was performed to determine the biomarker sensitivity/specificity. Low podocyte density and increased Mindin expression were observed in all cases of DN, regardless of their class. In the DN group, Mindin expression was significantly higher than that in the FSGS, MCD, IgAN and control groups. Higher Mindin expression was significantly positively correlated with foot process effacement only in class III DN cases. Furthermore, Mindin protein presented high specificity in the biopsies of patients with DN (p < 0.0001). Our data suggest that Mindin may play a role in DN pathogenesis and is a promising biomarker of podocyte lesions.
Identifiants
pubmed: 37130106
doi: 10.1371/journal.pone.0284789
pii: PONE-D-22-31916
pmc: PMC10153717
doi:
Substances chimiques
mindin
0
Biomarkers
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0284789Informations de copyright
Copyright: © 2023 Martins et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Kidney Int. 2001 Sep;60(3):957-68
pubmed: 11532090
Contrib Nephrol. 2011;170:1-7
pubmed: 21659752
J Clin Invest. 2001 Jul;108(2):289-301
pubmed: 11457882
Saudi J Kidney Dis Transpl. 2020 Sep-Oct;31(5):1014-1024
pubmed: 33229764
Diab Vasc Dis Res. 2021 Nov-Dec;18(6):14791641211058856
pubmed: 34791910
J Hepatol. 2015 Nov;63(5):1198-211
pubmed: 26165142
Hum Mol Genet. 2002 Mar 15;11(6):651-9
pubmed: 11912180
Am J Kidney Dis. 2018 Jun;71(6):884-895
pubmed: 29398179
J Am Coll Cardiol. 2009 Feb 3;53(5 Suppl):S35-42
pubmed: 19179216
Kidney Int. 2004 Nov;66(5):1901-6
pubmed: 15496161
Nephrol Dial Transplant. 2011 Jul;26(7):2153-60
pubmed: 21098016
Saudi J Kidney Dis Transpl. 2017 Jul-Aug;28(4):836-841
pubmed: 28748886
Diabetologia. 2005 Nov;48(11):2241-7
pubmed: 16170513
Diabetes. 2003 Apr;52(4):1023-30
pubmed: 12663475
Curr Opin Nephrol Hypertens. 2015 May;24(3):239-44
pubmed: 26066473
Kidney Int. 2010 Jun;77(11):989-99
pubmed: 20375978
J Cell Sci. 2004 Jul 1;117(Pt 15):3281-94
pubmed: 15226400
Infect Immun. 2000 Apr;68(4):1975-9
pubmed: 10722591
PLoS One. 2020 Mar 4;15(3):e0229765
pubmed: 32130282
BMC Nephrol. 2020 Jul 28;21(1):308
pubmed: 32723296
Kidney Int Suppl. 2007 Aug;(106):S36-42
pubmed: 17653209
Int J Biochem Cell Biol. 2010 Sep;42(9):1380-7
pubmed: 20542138
Blood. 2005 Dec 1;106(12):3854-9
pubmed: 16105980
J Am Soc Nephrol. 2003 Aug;14(8):2063-71
pubmed: 12874460
Kidney Int. 2006 Mar;69(5):806-14
pubmed: 16421517
Clin Sci (Lond). 2017 May 1;131(9):833-846
pubmed: 28424377
PLoS One. 2020 Nov 4;15(11):e0241745
pubmed: 33147279
Clin Sci (Lond). 2018 Jun 20;132(11):1199-1213
pubmed: 29695588
J Am Soc Nephrol. 2014 May;25(5):1118-29
pubmed: 24357669
Nephrology (Carlton). 2015 Oct;20(10):721-726
pubmed: 25973958
PLoS One. 2016 Jan 26;11(1):e0147329
pubmed: 26812415
J Am Soc Nephrol. 2010 Apr;21(4):556-63
pubmed: 20167701
Am J Physiol Renal Physiol. 2009 Sep;297(3):F594-603
pubmed: 19605546
N Engl J Med. 2008 Jun 12;358(24):2560-72
pubmed: 18539916
J Matern Fetal Neonatal Med. 2011 Oct;24 Suppl 2:44-7
pubmed: 21888469
Dev Biol. 1997 Dec 15;192(2):211-27
pubmed: 9441663
PLoS One. 2019 Jun 12;14(6):e0217569
pubmed: 31188898
Nephrol Dial Transplant. 2021 Jul 23;36(8):1384-1386
pubmed: 31883322