Single-cell DNA Methylome and 3D Multi-omic Atlas of the Adult Mouse Brain.


Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
18 Apr 2023
Historique:
pubmed: 3 5 2023
medline: 3 5 2023
entrez: 3 5 2023
Statut: epublish

Résumé

Cytosine DNA methylation is essential in brain development and has been implicated in various neurological disorders. A comprehensive understanding of DNA methylation diversity across the entire brain in the context of the brain's 3D spatial organization is essential for building a complete molecular atlas of brain cell types and understanding their gene regulatory landscapes. To this end, we employed optimized single-nucleus methylome (snmC-seq3) and multi-omic (snm3C-seq

Identifiants

pubmed: 37131654
doi: 10.1101/2023.04.16.536509
pmc: PMC10153407
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIH HHS
ID : S10 OD023689
Pays : United States
Organisme : NIMH NIH HHS
ID : U19 MH114830
Pays : United States
Organisme : NIMH NIH HHS
ID : U19 MH114831
Pays : United States

Déclaration de conflit d'intérêts

Competing Interests J.R.E serves on the scientific advisory board of Zymo Research Inc. B.R. is a shareholder of Arima Genomics Inc., and Epigenome Technologies, Inc. H.Z. is on the scientific advisory board of MapLight Therapeutics, Inc

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Auteurs

Hanqing Liu (H)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Qiurui Zeng (Q)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.
Division of Biological Sciences, University of California, San Diego, La Jolla, CA, USA.

Jingtian Zhou (J)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.
Bioinformatics and Systems Biology Program, University of California, San Diego, La Jolla, CA, USA.

Anna Bartlett (A)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Bang-An Wang (BA)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Peter Berube (P)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.
Division of Biological Sciences, University of California, San Diego, La Jolla, CA, USA.

Wei Tian (W)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Mia Kenworthy (M)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Jordan Altshul (J)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Joseph R Nery (JR)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Huaming Chen (H)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Rosa G Castanon (RG)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Songpeng Zu (S)

Department of Cellular and Molecular Medicine, University of California, San Diego School of Medicine, La Jolla, CA, USA.

Yang Eric Li (YE)

Department of Cellular and Molecular Medicine, University of California, San Diego School of Medicine, La Jolla, CA, USA.

Jacinta Lucero (J)

Computational Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Julia K Osteen (JK)

Computational Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Antonio Pinto-Duarte (A)

Computational Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Jasper Lee (J)

Computational Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Jon Rink (J)

Computational Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Silvia Cho (S)

Computational Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Nora Emerson (N)

Computational Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Michael Nunn (M)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Carolyn O'Connor (C)

Flow Cytometry Core Facility, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Zizhen Yao (Z)

Allen Institute for Brain Science, Seattle, WA, USA.

Kimberly A Smith (KA)

Allen Institute for Brain Science, Seattle, WA, USA.

Bosiljka Tasic (B)

Allen Institute for Brain Science, Seattle, WA, USA.

Hongkui Zeng (H)

Allen Institute for Brain Science, Seattle, WA, USA.

Chongyuan Luo (C)

Department of Human Genetics, University of California Los Angeles, Los Angeles, CA, USA.

Jesse R Dixon (JR)

Peptide Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Bing Ren (B)

Department of Cellular and Molecular Medicine, University of California, San Diego School of Medicine, La Jolla, CA, USA.
Center for Epigenomics, University of California, San Diego School of Medicine, La Jolla, CA, USA.
Institute of Genomic Medicine, University of California, San Diego School of Medicine, La Jolla, CA, USA.

M Margarita Behrens (MM)

Computational Neurobiology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Joseph R Ecker (JR)

Genomic Analysis Laboratory, The Salk Institute for Biological Studies, La Jolla, CA, USA.
Howard Hughes Medical Institute, The Salk Institute for Biological Studies, La Jolla, CA, USA.

Classifications MeSH