Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat production.

Autocrine signaling Cell line engineering Cellular agriculture Cultivated meat Cultured meat Fibroblast growth factor Satellite cells Serum-free media

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
17 Apr 2023
Historique:
pubmed: 3 5 2023
medline: 3 5 2023
entrez: 3 5 2023
Statut: epublish

Résumé

Cultured meat is a promising technology that faces substantial cost barriers which are currently driven largely by the price of media components. Growth factors such as fibroblast growth factor 2 (FGF2) drive the cost of serum-free media for relevant cells including muscle satellite cells. Here, we engineered immortalized bovine satellite cells (iBSCs) for inducible expression of FGF2 and/or mutated Ras

Identifiants

DOI: 10.1101/2023.04.17.537163 PMID: 37131805 PMC: PMC10153192
pubmed: 37131805
doi: 10.1101/2023.04.17.537163
pmc: PMC10153192
pii:
doi:

Types de publication

Preprint

Langues

eng

Déclaration de conflit d'intérêts

Declaration of competing interests The authors declare no competing interests.

Auteurs

Andrew J Stout (AJ)

Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA.

Xiaoli Zhang (X)

Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA.

Sophia M Letcher (SM)

Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA.

Miriam L Rittenberg (ML)

Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA.
Biological Engineering Department, Massachusetts Institute of Technology, Cambridge, MA, USA.

Michelle Shub (M)

Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA.

Kristin M Chai (KM)

Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA.

Maya Kaul (M)

Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA.

David L Kaplan (DL)

Biomedical Engineering Department, Tissue Engineering Resource Center, Tufts University, Medford, MA, USA.

Classifications MeSH