Genetic Mouse Models of Pneumocystis Pneumonia.
Immune response
Immunocompromised
Lung fungal infection
Pneumocystis murina
Rag1-deficient
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
medline:
8
5
2023
pubmed:
5
5
2023
entrez:
5
5
2023
Statut:
ppublish
Résumé
Pneumocystis jirovecii causes pneumonia in immunocompromised patients. A major challenge in drug susceptibility testing and in understanding host/pathogen interactions is that Pneumocystis spp. are not viable in vitro. Continuous culture of the organism is not currently available, and therefore, developing new drug targets is very limited. Due to this limitation, mouse models of Pneumocystis pneumonia have proven to be an invaluable resource to researchers. In this chapter, we provide an overview of selected methods used in mouse models of infection including, in vivo Pneumocystis murina propagation, routes of transmission, genetic mouse models available, a P. murina life form-specific model, a mouse model of PCP immune reconstitution inflammatory syndrome (IRIS), and the experimental parameters associated with these models.
Identifiants
pubmed: 37145284
doi: 10.1007/978-1-0716-3199-7_13
doi:
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
169-179Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Ma L, Chen Z, Huang D et al (2016) Genome analysis of three Pneumocystis species reveals adaptation mechanisms to life exclusively in mammalian hosts. Nat Commun 7:10740
doi: 10.1038/ncomms10740
pubmed: 26899007
pmcid: 4764891
Cushion MT, Tisdale-Macioce N, Sayson SG et al (2021) The persistent challenge of Pneumocystis growth outside the mammalian lung: Past and future approaches. Front Microbiol 12:681474
doi: 10.3389/fmicb.2021.681474
pubmed: 34093506
pmcid: 8174303
Shellito J, Suzara VV, Blumenfeld W et al (1990) A new model of Pneumocystis carinii infection in mice selectively depleted of helper T lymphocytes. J Clin Invest 85:16861693
doi: 10.1172/JCI114621
Hughes WT (1982) Natural mode of acquisition for de novo infection with Pneumocystis carinii. J Infect Dis 145:842–848
doi: 10.1093/infdis/145.6.842
pubmed: 6979590
Powles MA, McFadden DC, Pittarelli LA et al (1992) Mouse model for Pneumocystis carinii pneumonia that uses natural transmission to initiate infection. Infect Immun 4:1397–1400
doi: 10.1128/iai.60.4.1397-1400.1992
Wolff L, Horch S, Gemsa D (1993) The development of Pneumocystis Carinii Pneumonia in germ-free rats requires immunosuppression and exposure to the Pneumocystis carinii organism. Comp Immunol Microbiol Infect Dis 16:73–76
doi: 10.1016/0147-9571(93)90063-B
pubmed: 8440087
An CL, Gigliotti F, Harmsen AG (2003) Exposure of immunocompetent Adult Mice to Pneumocystis carinii f. Sp. Muris by Cohousing: Growth of P. Carinii f. Sp. Muris and Host immune response. Infect Immun 71:2065–2070
doi: 10.1128/IAI.71.4.2065-2070.2003
pubmed: 12654827
pmcid: 152044
Chesnay A, Paget C, Heuzé-Vourc'h N et al (2022) Pneumocystis Pneumonia: pitfalls and hindrances to establishing a reliable animal model. J Fungi (Basel) 8:129
doi: 10.3390/jof8020129
pubmed: 35205883
Evans HM, Garvy BA (2018) The trophic life cycle stage of Pneumocystis species induces protective adaptive responses without inflammation-mediated progression to pneumonia. Med Mycol 56:994–1005
pubmed: 29267980
Cushion MT, Linke MJ, Ashbaugh A et al (2010) Echinocandin treatment of pneumocystis pneumonia in rodent models depletes cysts leaving trophic burdens that cannot transmit the infection. PLoS One 5:e8524
doi: 10.1371/journal.pone.0008524
pubmed: 20126455
pmcid: 2813285
Wright TW, Gigliotti F, Finkelstein JN et al (1999) Immune-mediated inflammation directly impairs pulmonary function, contributing to the pathogenesis of Pneumocystis carinii pneumonia. J Clin Invest 104:1307–1317
doi: 10.1172/JCI6688
pubmed: 10545529
pmcid: 409816
Barry SM, Lipman MC, Deery AR et al (2002) Immune reconstitution pneumonitis following Pneumocystis carinii pneumonia in HIV infected subjects. HIV Med 3:207–211
doi: 10.1046/j.1468-1293.2002.00115.x
pubmed: 12139660
Bhagwat SP, Gigliotti F, Xu H et al (2006) Contribution of T cell subsets to the pathophysiology of Pneumocystis-related immunorestitution disease. Am J Physiol Lung Cell Mol Physiol 291:L1256–L1266
doi: 10.1152/ajplung.00079.2006
pubmed: 16891394
Wang J, Gigliotti F, Bhagwat SP et al (2010) Immune modulation with sulfasalazine attenuates immunopathogenesis but enhances macrophage-mediated fungal clearance during Pneumocystis pneumonia. PLoS Pathog 19:e1001058
doi: 10.1371/journal.ppat.1001058
Hanano R, Reifenberg K, Kaufmann SH (1996) Naturally acquired pneumocystis carinii pneumonia in gene disruption mutant mice: roles of distinct T-cell populations in infection. Infect Immun 64:3201–3209
doi: 10.1128/iai.64.8.3201-3209.1996
pubmed: 8757854
pmcid: 174208
Zheng M, Cai Y, Eddens T et al (2014) Novel pneumocystis antigen discovery using fungal surface proteomics. Infect Immun 82:2417–2423
doi: 10.1128/IAI.01678-13
pubmed: 24686066
pmcid: 4019171
McKinley L, Logar AJ, McAllister F et al (2006) Regulatory T cells dampen pulmonary inflammation and lung injury in an animal model of pneumocystis pneumonia. J Immunol 177:6215–6226
doi: 10.4049/jimmunol.177.9.6215
pubmed: 17056551
Elsegeiny W, Zheng M, Eddens T et al (2018) Murine models of pneumocystis infection recapitulate human primary immune disorders. JCI Insight 21:e91894
doi: 10.1172/jci.insight.91894
Zheng M, Shellito JE, Marrero L et al (2001) CD4+ T cell-independent vaccination against Pneumocystis carinii in mice. J Clin Invest 108:1469–1474
doi: 10.1172/JCI13826
pubmed: 11714738
pmcid: 209424
Ricks DM, Chen K, Zheng M et al (2013) Dectin immunoadhesins and pneumocystis pneumonia. Infect Immun 81:3451–3462
doi: 10.1128/IAI.00136-13
pubmed: 23836814
pmcid: 3754224
Eddens T, Elsegeiny W, Nelson MP et al (2015) Eosinophils contribute to early clearance of Pneumocystis murina infection. J Immunol 195:185–193
doi: 10.4049/jimmunol.1403162
pubmed: 25994969
Nature (2002) Initial sequencing and comparative analysis of the mouse genome. Nature 420:520–562
doi: 10.1038/nature01262
Kelly MN, Zheng M, Ruan S et al (2013) Memory CD4+T cells are required for optimal NK cell effector functions against the opportunistic fungal pathogen Pneumocystis murina. J Immunol 190:285–295
doi: 10.4049/jimmunol.1200861
pubmed: 23203926
Opata MM, Hollifield ML, Lund FE et al (2015) B lymphocytes are required during the early priming of CD4+ T cells for clearance of Pneumocystis infection in mice. J Immunol 195:611–620
doi: 10.4049/jimmunol.1500112
pubmed: 26041535
Wright TW, Johnston CJ, Harmsen AG et al (1999) Chemokine gene expression during Pneumocystis carinii-driven pulmonary inflammation. Infect Immun 67:3452–3460
doi: 10.1128/IAI.67.7.3452-3460.1999
pubmed: 10377126
pmcid: 116531
Roths JB, Marshall JD, Allen RD (1990) Spontaneous Pneumocystis carinii pneumonia in immunodeficient mutant scid mice. Natural history and pathobiology. Am J Pathol 136:1173
pubmed: 2349968
pmcid: 1877432
Lund FE, Schuer K, Hollifield ML et al (2003) Clearance of pneumocystis carinii in mice is dependent on B cells but not on P. carinii-specific antibody. J Immunol 171:1423–1430
doi: 10.4049/jimmunol.171.3.1423
pubmed: 12874234
Wiley J, Harmsen A (1995) CD40 ligand is required for resolution of Pneumocystis carinii pneumonia in mice. J Immunol 155:3525–3529
doi: 10.4049/jimmunol.155.7.3525
pubmed: 7561048
Linke MJ, Ashbaugh AD, Demland JA et al (2009) Pneumocystis murina colonization in immunocompetent surfactant protein a deficient mice following environmental exposure. Respir Res 10:1–15
doi: 10.1186/1465-9921-10-10
Tasaka S, Hasegawa N, Kobayashi S et al (2007) Serum indicators for the diagnosis of pneumocystis pneumonia. Chest 131:1173–1180
doi: 10.1378/chest.06-1467
pubmed: 17426225
Linke MJ, Ashbaugh A, Collins MS et al (2013) Characterization of a distinct host response profile to Pneumocystis murina asci during clearance of pneumocystis pneumonia. Infect Immun 81:984–995
doi: 10.1128/IAI.01181-12
pubmed: 23319554
pmcid: 3584873
Cushion MT, Collins MS, Linke MJ (2009) Biofilm formation by Pneumocystis spp. Eukaryot Cell 8:197–206
doi: 10.1128/EC.00202-08
pubmed: 18820078
Odabasi Z, Paetznick VL, Rodriguez JR et al (2006) Differences in beta-glucan levels in culture supernatants of a variety of fungi. Med Mycol 44:267–272
doi: 10.1080/13693780500474327
pubmed: 16702107