Single-cell analysis of the postnatal dorsal V-SVZ reveals a role for Bmpr1a signaling in silencing pallial germinal activity.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
05 05 2023
05 05 2023
Historique:
medline:
8
5
2023
pubmed:
5
5
2023
entrez:
5
5
2023
Statut:
ppublish
Résumé
The ventricular-subventricular zone (V-SVZ) is the largest neurogenic region of the postnatal forebrain, containing neural stem cells (NSCs) that emerge from both the embryonic pallium and subpallium. Despite of this dual origin, glutamatergic neurogenesis declines rapidly after birth, while GABAergic neurogenesis persists throughout life. We performed single-cell RNA sequencing of the postnatal dorsal V-SVZ for unraveling the mechanisms leading to pallial lineage germinal activity silencing. We show that pallial NSCs enter a state of deep quiescence, characterized by high bone morphogenetic protein (BMP) signaling, reduced transcriptional activity and Hopx expression, while in contrast, subpallial NSCs remain primed for activation. Induction of deep quiescence is paralleled by a rapid blockade of glutamatergic neuron production and differentiation. Last, manipulation of Bmpr1a demonstrates its key role in mediating these effects. Together, our results highlight a central role of BMP signaling in synchronizing quiescence induction and blockade of neuronal differentiation to rapidly silence pallial germinal activity after birth.
Identifiants
pubmed: 37146152
doi: 10.1126/sciadv.abq7553
pmc: PMC10162676
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
eabq7553Références
J Comp Neurol. 2003 Dec 1;467(1):60-79
pubmed: 14574680
Cell Stem Cell. 2012 Aug 3;11(2):220-30
pubmed: 22862947
Nat Neurosci. 2015 May;18(5):657-65
pubmed: 25821910
Nat Neurosci. 2009 Dec;12(12):1524-33
pubmed: 19881504
Stem Cells. 2007 Jan;25(1):63-8
pubmed: 16973830
Elife. 2021 Jul 14;10:
pubmed: 34259628
Cell Rep. 2020 Mar 31;30(13):4490-4504.e4
pubmed: 32234482
J Neurosci. 2007 Aug 1;27(31):8286-96
pubmed: 17670975
Cell Rep. 2018 Mar 6;22(10):2567-2574
pubmed: 29514086
Science. 1999 Apr 16;284(5413):479-82
pubmed: 10205054
Science. 2015 Jun 26;348(6242):aaa6071
pubmed: 26113728
Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3299-304
pubmed: 12626757
J Neurosci. 2010 Jul 21;30(29):9771-81
pubmed: 20660259
Nature. 2018 Aug;560(7719):494-498
pubmed: 30089906
Elife. 2019 Sep 25;8:
pubmed: 31552825
Proc Natl Acad Sci U S A. 2009 Apr 14;106(15):6387-92
pubmed: 19332781
Cell Rep. 2018 Nov 27;25(9):2457-2469.e8
pubmed: 30485812
Neuron. 2014 Dec 3;84(5):927-39
pubmed: 25467979
Bioinformatics. 2019 Sep 1;35(17):3038-3045
pubmed: 30649203
J Neurosci. 2007 Jun 27;27(26):6878-91
pubmed: 17596436
Nature. 2013 Jan 10;493(7431):226-30
pubmed: 23201681
Development. 2015 Jun 15;142(12):2109-20
pubmed: 26081572
Cell. 2018 Aug 9;174(4):999-1014.e22
pubmed: 30096314
Proc Natl Acad Sci U S A. 2014 Aug 26;111(34):12438-43
pubmed: 25114218
OMICS. 2012 May;16(5):284-7
pubmed: 22455463
Cell Rep. 2019 Jan 8;26(2):394-406.e5
pubmed: 30625322
Cell Rep. 2017 Aug 29;20(9):2144-2155
pubmed: 28854364
J Histochem Cytochem. 2007 Jun;55(6):545-54
pubmed: 17242468
Sci Adv. 2021 Dec 10;7(50):eabj1598
pubmed: 34878841
PLoS Biol. 2017 Mar 28;15(3):e2000698
pubmed: 28350803
Cell. 2018 Jul 26;174(3):716-729.e27
pubmed: 29961576
Stem Cell Reports. 2015 Oct 13;5(4):461-70
pubmed: 26411905
Nat Methods. 2017 Nov;14(11):1083-1086
pubmed: 28991892
Stem Cell Res. 2015 Nov;15(3):522-529
pubmed: 26451648
Nat Commun. 2021 May 10;12(1):2594
pubmed: 33972529
Cereb Cortex. 2013 Apr;23(4):922-31
pubmed: 22473896
STAR Protoc. 2020 Jun 19;1(1):100027
pubmed: 32685931
Cell. 2014 Nov 6;159(4):775-88
pubmed: 25417155
Cell. 2019 Apr 18;177(3):654-668.e15
pubmed: 30929900
Science. 2011 Dec 9;334(6061):1420-4
pubmed: 22075725
Cell Rep. 2017 Aug 15;20(7):1597-1608
pubmed: 28813672
Front Cell Neurosci. 2012 Feb 27;6:6
pubmed: 22371698
EMBO J. 2016 May 2;35(9):924-41
pubmed: 26856890
Cell Rep. 2020 Jun 23;31(12):107805
pubmed: 32579931
Stem Cell Reports. 2018 Sep 11;11(3):770-783
pubmed: 30174314
Nat Neurosci. 2010 Nov;13(11):1365-72
pubmed: 20935640
Neural Dev. 2011 Apr 05;6:12
pubmed: 21466690
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15545-50
pubmed: 16199517
Genome Res. 2015 Dec;25(12):1860-72
pubmed: 26430063
PLoS One. 2012;7(11):e49087
pubmed: 23166605
Proc Natl Acad Sci U S A. 2019 Apr 2;116(14):7089-7094
pubmed: 30894491
BMC Genomics. 2020 Jul 14;21(1):482
pubmed: 32664861
J Cell Sci. 2010 Jun 1;123(Pt 11):1922-30
pubmed: 20460439
Cereb Cortex. 2008 Mar;18(3):553-62
pubmed: 17576749
Dev Cell. 2010 Sep 14;19(3):450-9
pubmed: 20833366
Cell. 2019 Jun 13;177(7):1888-1902.e21
pubmed: 31178118
Nat Commun. 2019 Jan 11;10(1):134
pubmed: 30635555
Stem Cells. 2014 May;32(5):1301-12
pubmed: 24449255
Neuron. 2017 Jul 19;95(2):309-325.e6
pubmed: 28728023
Am J Pathol. 2011 Dec;179(6):2920-30
pubmed: 21996676
Cell Stem Cell. 2015 Sep 3;17(3):329-40
pubmed: 26235341
Science. 2005 Jun 24;308(5730):1927-30
pubmed: 15976303
Stem Cell Reports. 2019 Oct 8;13(4):700-712
pubmed: 31543472
Neuron. 2014 May 7;82(3):545-59
pubmed: 24811379
Cell. 2015 Jun 18;161(7):1644-55
pubmed: 26091041
Oncogene. 2020 Jul;39(28):5112-5123
pubmed: 32533098
Stem Cells. 2015 Jul;33(7):2232-42
pubmed: 25827345
Cell Stem Cell. 2010 Jul 2;7(1):78-89
pubmed: 20621052
Elife. 2020 Aug 07;9:
pubmed: 32762844