Characterization of RTgill-W1 cells epithelial development on transwell inserts: Evaluation of osmotic and toxic challenges.
Animal alternatives
Aquatic toxicity
Cytotoxicity assays
RTgill-W1 cells
Transwell inserts
Journal
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
ISSN: 1532-0456
Titre abrégé: Comp Biochem Physiol C Toxicol Pharmacol
Pays: United States
ID NLM: 100959500
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
19
02
2023
revised:
11
04
2023
accepted:
26
04
2023
medline:
2
6
2023
pubmed:
7
5
2023
entrez:
6
5
2023
Statut:
ppublish
Résumé
RTgill-W1 cells cannot be directly exposed to freshwater (FW) or seawater (SW) due to osmotic stress. Adjustments of exposure solutions are needed, but these might reduce the bioavailability and toxicity of pollutants. To facilitate cell polarization and allow direct exposure of water samples, cells were cultured on transwell inserts. Monolayer formation was measured by trans-epithelial electrical resistance (TEER) and an apparent permeability (P
Identifiants
pubmed: 37149014
pii: S1532-0456(23)00093-5
doi: 10.1016/j.cbpc.2023.109638
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109638Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest This manuscript has not been published and is not under consideration for publication elsewhere. We have no conflicts of interest to disclose.