Nanoscale and functional heterogeneity of the hippocampal extracellular space.
CP: Neuroscience
brain
extracellular matrix
extracellular space
immunoglobulin
single molecule
single quantum dot imaging
super-resolution
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
30 05 2023
30 05 2023
Historique:
received:
20
05
2022
revised:
17
02
2023
accepted:
20
04
2023
medline:
5
6
2023
pubmed:
7
5
2023
entrez:
7
5
2023
Statut:
ppublish
Résumé
The extracellular space (ECS) and its constituents play a crucial role in brain development, plasticity, circadian rhythm, and behavior, as well as brain diseases. Yet, since this compartment has an intricate geometry and nanoscale dimensions, its detailed exploration in live tissue has remained an unmet challenge. Here, we used a combination of single-nanoparticle tracking and super-resolution microscopy approaches to map the nanoscale dimensions of the ECS across the rodent hippocampus. We report that these dimensions are heterogeneous between hippocampal areas. Notably, stratum radiatum CA1 and CA3 ECS differ in several characteristics, a difference that gets abolished after digestion of the extracellular matrix. The dynamics of extracellular immunoglobulins vary within these areas, consistent with their distinct ECS characteristics. Altogether, we demonstrate that ECS nanoscale anatomy and diffusion properties are widely heterogeneous across hippocampal areas, impacting the dynamics and distribution of extracellular molecules.
Identifiants
pubmed: 37149864
pii: S2211-1247(23)00489-8
doi: 10.1016/j.celrep.2023.112478
pmc: PMC10242443
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
112478Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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