Cytological, molecular, cytogenetic, and physiological characterization of a novel immortalized human enteric glial cell line.

enteric glial cells enteric nervous system immortalized human cell line transgene immortalization viral transduction

Journal

Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935

Informations de publication

Date de publication:
2023
Historique:
received: 21 02 2023
accepted: 22 03 2023
medline: 8 5 2023
pubmed: 8 5 2023
entrez: 8 5 2023
Statut: epublish

Résumé

Enteric glial cells (EGCs), the major components of the enteric nervous system (ENS), are implicated in the maintenance of gut homeostasis, thereby leading to severe pathological conditions when impaired. However, due to technical difficulties associated with EGCs isolation and cell culture maintenance that results in a lack of valuable

Identifiants

pubmed: 37153631
doi: 10.3389/fncel.2023.1170309
pmc: PMC10158601
doi:

Types de publication

Journal Article

Langues

eng

Pagination

1170309

Informations de copyright

Copyright © 2023 Zanoletti, Valdata, Nehlsen, Faris, Casali, Cacciatore, Sbarsi, Carriero, Arfini, van Baarle, De Simone, Barbieri, Raimondi, May, Moccia, Bozzola, Matteoli, Comincini and Manai.

Déclaration de conflit d'intérêts

TM holds a patent for the immortalization technology described in this manuscript and is a shareholder of InSCREENeX GmbH, which commercializes cell lines immortalized by the described technology. TM and KN are employees of InSCREENeX GmbH. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Références

Cancer Biol Ther. 2013 Jul;14(7):574-86
pubmed: 23792642
Nat Commun. 2018 Mar 8;9(1):994
pubmed: 29520052
Gastroenterology. 2018 Aug;155(2):245-251
pubmed: 29964042
Cell Stress. 2019 May 21;3(6):165-180
pubmed: 31225512
Neurogastroenterol Motil. 2005 Dec;17(6):777-90
pubmed: 16336493
Am J Physiol Gastrointest Liver Physiol. 2012 Oct 15;303(8):G887-93
pubmed: 22878122
Clin Gastroenterol Hepatol. 2007 Dec;5(12):1406-12
pubmed: 17919990
J Exp Clin Cancer Res. 2018 Nov 1;37(1):266
pubmed: 30382874
Prog Neurobiol. 2011 Mar;93(3):421-43
pubmed: 21219963
J Neurosci. 2015 Feb 18;35(7):3016-21
pubmed: 25698739
Neurogastroenterol Motil. 2001 Feb;13(1):95-106
pubmed: 11169131
Gastroenterology. 2007 Apr;132(4):1344-58
pubmed: 17408650
J Gastroenterol Hepatol. 2018 Mar;33(3):638-644
pubmed: 28873259
Nat Rev Gastroenterol Hepatol. 2021 Aug;18(8):571-587
pubmed: 33731961
J Anat. 1972 Jan;111(Pt 1):69-97
pubmed: 4335909
Hum Pathol. 2011 Apr;42(4):522-32
pubmed: 21208643
Cell Tissue Res. 1994 Oct;278(1):153-60
pubmed: 7954696
J Physiol. 2017 Jan 15;595(2):583-598
pubmed: 27436013
Sci Rep. 2015 Mar 20;5:9226
pubmed: 25791532
Lab Invest. 2007 Jul;87(7):628-32
pubmed: 17483847
Chromosome Res. 2012 Jul;20(5):465-79
pubmed: 22825424
Neurogastroenterol Motil. 2013 Feb;25(2):e151-60
pubmed: 23279281
Cell Mol Gastroenterol Hepatol. 2016 Jan 1;2(1):77-91
pubmed: 26771001
J Neuroimmunol. 2001 Jul 2;117(1-2):9-20
pubmed: 11431000
Nat Commun. 2018 Jul 27;9(1):2961
pubmed: 30054475
Curr Opin Immunol. 2022 Aug;77:102183
pubmed: 35533467
J Neurosci. 1983 Nov;3(11):2206-18
pubmed: 6138397
Cancers (Basel). 2020 Nov 11;12(11):
pubmed: 33187307
Glia. 2003 May;42(3):252-62
pubmed: 12673831
Cell Mol Life Sci. 2020 Jun;77(11):2235-2253
pubmed: 31473770
Gut. 2004 Feb;53(2):222-8
pubmed: 14724154
BMC Gastroenterol. 2011 Jan 14;11:3
pubmed: 21235736
Sci Signal. 2019 May 21;12(582):
pubmed: 31113852
Neurogastroenterol Motil. 2009 Nov;21(11):1209-e112
pubmed: 19558426
Glia. 2019 Jun;67(6):1167-1178
pubmed: 30730592
Neurogastroenterol Motil. 2009 Aug;21(8):870-e62
pubmed: 19368656
Nat Protoc. 2008;3(6):1101-8
pubmed: 18546601
Gastroenterology. 2009 Apr;136(4):1349-58
pubmed: 19250649
Clin Cancer Res. 2005 Apr 1;11(7):2747-55
pubmed: 15814657
Cells. 2020 Mar 31;9(4):
pubmed: 32244316
World J Stem Cells. 2015 Mar 26;7(2):470-6
pubmed: 25815130
Nat Med. 2000 May;6(5):513-9
pubmed: 10802706
PLoS One. 2016 Mar 10;11(3):e0151335
pubmed: 26964064
J Neurochem. 2017 Sep;142(6):876-885
pubmed: 28677119
J Neurosci. 2010 May 12;30(19):6801-9
pubmed: 20463242
Physiol Rev. 2015 Oct;95(4):1383-436
pubmed: 26400989
Nature. 2016 Jul 21;535(7612):440-443
pubmed: 27409807
J Vis Exp. 2018 Aug 15;(138):
pubmed: 30175991
EBioMedicine. 2019 Nov;49:172-188
pubmed: 31662289
Gastroenterology. 1992 Aug;103(2):439-47
pubmed: 1378803
Cell Mol Neurobiol. 2021 Apr;41(3):589
pubmed: 32535721
Cells. 2022 Aug 11;11(16):
pubmed: 36010578
PLoS One. 2011;6(10):e26889
pubmed: 22046396
Inflamm Bowel Dis. 2016 Aug;22(8):1812-34
pubmed: 27416040
Am J Physiol. 1997 May;272(5 Pt 1):G1175-85
pubmed: 9176228
Nature. 2008 Aug 28;454(7208):1046-8
pubmed: 18756229
J Comp Neurol. 2008 Aug 1;509(4):356-71
pubmed: 18512230
J Clin Pathol. 2021 Sep;74(9):548-552
pubmed: 34039664
Neurogastroenterol Motil. 2013 Nov;25(11):e755-64
pubmed: 23991747
Inflamm Bowel Dis. 2016 Feb;22(2):433-49
pubmed: 26689598
Am J Physiol. 1998 Sep;275(3):G550-5
pubmed: 9724268
J Pers Med. 2021 Sep 22;11(10):
pubmed: 34683083
Neurogastroenterol Motil. 2004 Apr;16 Suppl 1:44-9
pubmed: 15066004
J Vasc Res. 2004 Mar-Apr;41(2):166-73
pubmed: 15004436
Methods. 2008 Nov;46(3):204-12
pubmed: 18929662
Front Syst Neurosci. 2017 Oct 23;11:79
pubmed: 29109680
Am J Physiol Gastrointest Liver Physiol. 2018 Jul 1;315(1):G1-G11
pubmed: 29517926
Proc Natl Acad Sci U S A. 2018 May 29;115(22):5798-5803
pubmed: 29760072
Trends Neurosci. 2015 Jun;38(6):364-74
pubmed: 25975510
Arterioscler Thromb Vasc Biol. 2007 Dec;27(12):2514-23
pubmed: 17872453
J Physiol. 2017 Jun 1;595(11):3409-3424
pubmed: 28066889
Cell. 1998 Apr 17;93(2):189-201
pubmed: 9568712
Neurogastroenterol Motil. 2001 Feb;13(1):89-94
pubmed: 11169130
Nature. 1980 Aug 14;286(5774):736-7
pubmed: 6997753
Neurosci Bull. 2014 Feb;30(1):125-33
pubmed: 23677809
Neuroscience. 1981;6(3):425-36
pubmed: 7219723
Am J Pathol. 1987 Dec;129(3):493-502
pubmed: 3425689
World J Gastroenterol. 2011 Mar 14;17(10):1261-6
pubmed: 21455324
Gastroenterology. 2020 Jan;158(1):189-199
pubmed: 31600487
Neurosci Res. 2017 Jun;119:15-23
pubmed: 28063977
Chromosoma. 2018 Jun;127(2):175-186
pubmed: 29322240
J Neurochem. 1996 Feb;66(2):604-12
pubmed: 8592130
Nat Rev Gastroenterol Hepatol. 2012 Nov;9(11):625-32
pubmed: 22890111
J Cell Sci. 2000 Oct;113 Pt 19:3365-74
pubmed: 10984428
Nat Neurosci. 2016 Dec;19(12):1619-1627
pubmed: 27775719
Neurogastroenterol Motil. 2011 Sep;23(9):e372-82
pubmed: 21762414
In Vitro Cell Dev Biol Anim. 2002 Apr;38(4):188-90
pubmed: 12197768
PLoS One. 2014 Mar 06;9(3):e91099
pubmed: 24603752

Auteurs

Lisa Zanoletti (L)

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia, Italy.
Department of Chronic Diseases and Metabolism (CHROMETA), KU Leuven, Leuven, Belgium.

Aurora Valdata (A)

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia, Italy.

Kristina Nehlsen (K)

InSCREENeX GmbH, Braunschweig, Germany.

Pawan Faris (P)

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia, Italy.
Department of Biology, College of Science, Salahaddin University-Erbil, Erbil, Iraq.

Claudio Casali (C)

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia, Italy.

Rosalia Cacciatore (R)

Immunohematology and Transfusion Service, I.R.C.C.S. Policlinico San Matteo, Pavia, Italy.

Ilaria Sbarsi (I)

Immunohematology and Transfusion Service, I.R.C.C.S. Policlinico San Matteo, Pavia, Italy.

Francesca Carriero (F)

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia, Italy.

Davide Arfini (D)

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia, Italy.

Lies van Baarle (L)

Department of Chronic Diseases and Metabolism (CHROMETA), KU Leuven, Leuven, Belgium.

Veronica De Simone (V)

Department of Chronic Diseases and Metabolism (CHROMETA), KU Leuven, Leuven, Belgium.

Giulia Barbieri (G)

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia, Italy.

Elena Raimondi (E)

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia, Italy.

Tobias May (T)

InSCREENeX GmbH, Braunschweig, Germany.

Francesco Moccia (F)

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia, Italy.

Mauro Bozzola (M)

University of Pavia, Pavia, Italy.

Gianluca Matteoli (G)

Department of Chronic Diseases and Metabolism (CHROMETA), KU Leuven, Leuven, Belgium.

Sergio Comincini (S)

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia, Italy.

Federico Manai (F)

Department of Biology and Biotechnology "L. Spallanzani", University of Pavia, Pavia, Italy.

Classifications MeSH