Heat-Denatured Lysozyme is a Novel Potential Non-alcoholic Disinfectant Against Respiratory Virus.
Humans
Animals
Mice
Muramidase
/ pharmacology
Disinfectants
/ pharmacology
SARS-CoV-2
Influenza A Virus, H1N1 Subtype
Influenza A Virus, H5N1 Subtype
Hot Temperature
Influenza A Virus, H7N1 Subtype
COVID-19
Influenza Pandemic, 1918-1919
Severe acute respiratory syndrome-related coronavirus
Antiviral Agents
/ pharmacology
Influenza A virus
Influenza, Human
Antiviral
Disinfectant
Heating-denatured
Influenza
Lysozyme
Journal
Food and environmental virology
ISSN: 1867-0342
Titre abrégé: Food Environ Virol
Pays: United States
ID NLM: 101483831
Informations de publication
Date de publication:
09 2023
09 2023
Historique:
received:
23
03
2022
accepted:
20
04
2023
medline:
14
9
2023
pubmed:
8
5
2023
entrez:
8
5
2023
Statut:
ppublish
Résumé
Respiratory diseases are significant recurrent threats to global public health. Since the 1918 Spanish flu pandemic, seasonal influenza viruses continue to cause epidemics around the world each year. More recently, the COVID-19 global pandemic conducted a public health crisis with more than 6 million deaths and it also severely affected the global economy. Due to the phenomenon that people get infection from objects carrying viruses, it has aroused people's attention to home disinfection. As there is no ideal existing common domestic disinfectant, new and safer antiviral disinfectants are urgently needed. Lysozyme is a natural antibacterial agent widespread in nature and widely used in healthcare and food industry because of is recognized safety. Recently, it has been shown that thermally denatured lysozyme has the ability to kill murine norovirus and hepatitis A virus. In our study, we also demonstrated that heat-denatured lysozyme (HDLz) had an antiviral effect against H1N1 influenza A virus, and we optimized its antiviral activities by testing different heating denaturation conditions, to generalize this property, using pseudotype virus neutralization assay, we found that HDLz can also inhibit the entry of H5N1, H5N6, and H7N1 avian influenza viruses as well as SARS-CoV and SARS-CoV-2 particles in cell with IC50 at the ng/mL range. Finally, using western blot analysis, we provide evidence that HDLz polymerization correlates with antiviral effect, which may be a precious possible quality control test. Altogether, our data support HDLz as a powerful anti-respiratory virus disinfectant as a sole or additive of current disinfectants to reduce concentration of toxic component.
Identifiants
pubmed: 37155116
doi: 10.1007/s12560-023-09556-1
pii: 10.1007/s12560-023-09556-1
pmc: PMC10166042
doi:
Substances chimiques
Muramidase
EC 3.2.1.17
Disinfectants
0
Antiviral Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
212-223Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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