ACT-107, a novel variant of AmpC β-lactamase from Enterobacter huaxiensis isolated from Neotropical leaf frog (Phyllomedusa distincta) inhabiting the Brazilian Atlantic Forest.

Humans Brazil beta-Lactamases / metabolism Cephalosporins
AmpC β-lactamase Antimicrobial resistance Brazilian Atlantic Forest Enterobacter Spp. Genomic surveillance

Journal

Journal of global antimicrobial resistance
ISSN: 2213-7173
Titre abrégé: J Glob Antimicrob Resist
Pays: Netherlands
ID NLM: 101622459

Informations de publication

Date de publication:
06 2023
Historique:
received: 08 10 2022
revised: 12 04 2023
accepted: 24 04 2023
medline: 20 6 2023
pubmed: 9 5 2023
entrez: 8 5 2023
Statut: ppublish

Résumé

The aim of this study was to characterise a broad-spectrum cephalosporin-resistant AmpC-positive Enterobacter huaxiensis colonising the skin of a Neotropical frog (Phyllomedusa distincta) inhabiting the Brazilian Atlantic Forest. During a genomic surveillance study of antimicrobial resistance, we screened skin samples from P. distincta. Gram-negative bacteria growing on MacConkey agar plates supplemented with 2 µg/mL ceftriaxone were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. A cephalosporin-resistant E. huaxiensis was sequenced using the Illumina NextSeq platform. Genomic data were analysed using bioinformatics tools, whereas AmpC β-lactamase was characterised in depth by comparative analysis of amino acids, in silico modelling, and analysis of susceptibility to β-lactam antibiotics and combinations of β-lactamase inhibitors. Whole-genome sequencing analysis revealed a novel variant of AmpC β-lactamase belonging to the ACT family, designated ACT-107 by NCBI. This variant contains 12 novel amino acid mutations within the ACT family, 5 in the signal peptide sequence (Ile2, Met14, Tyr16, Gly18 and Thr20), and 7 in the mature protein (Gln22, His43, Cys60, Thr157, Glu225, Ala252 and Asn310). In silico modelling showed that substitutions occurring in the mature chain are localised in the solvent-accessible surface of the protein, where they are not expected to affect the β-lactamase activity, as observed in the resistance profile. Strikingly, 'not designated' ACT variants from E. huaxiensis were clustered (> 96% identity) with ACT-107. Since E. huaxiensis has been isolated from human infection, ACT-107 requires surveillance and the attention of clinicians.

Identifiants

DOI: 10.1016/j.jgar.2023.04.016 PMID: 37156419 PMC: PMC10275763
pubmed: 37156419
pii: S2213-7165(23)00073-5
doi: 10.1016/j.jgar.2023.04.016
pmc: PMC10275763
pii:
doi:

Substances chimiques

AmpC beta-lactamases EC 3.5.2.6
beta-Lactamases EC 3.5.2.6
Cephalosporins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

353-359

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.

Déclaration de conflit d'intérêts

Competing interests None declared.

Références

Int J Syst Evol Microbiol. 2019 Mar;69(3):708-714
pubmed: 30614784
Nat Commun. 2018 Nov 30;9(1):5114
pubmed: 30504855
J Clin Microbiol. 2019 Feb 27;57(3):
pubmed: 30381421
Nucleic Acids Res. 2020 Jan 8;48(D1):D517-D525
pubmed: 31665441
Clin Microbiol Infect. 2017 Jan;23(1):2-22
pubmed: 27890457
PLoS Comput Biol. 2017 Jun 8;13(6):e1005595
pubmed: 28594827
Curr Protein Pept Sci. 2017;19(2):130-144
pubmed: 28745223
Clin Microbiol Rev. 2009 Jan;22(1):161-82, Table of Contents
pubmed: 19136439
Antimicrob Agents Chemother. 2015 Dec;59(12):7753-61
pubmed: 26438498
Antimicrob Agents Chemother. 2021 Nov 17;65(12):e0159621
pubmed: 34516244
J Chem Ecol. 2016 Feb;42(2):139-48
pubmed: 26826104
Microb Drug Resist. 2021 May;27(5):585-589
pubmed: 32991273
J Clin Microbiol. 2005 Dec;43(12):5945-9
pubmed: 16333080
Antimicrob Agents Chemother. 2021 Jun 17;65(7):e0242420
pubmed: 33903106
Methods Mol Biol. 2018;1685:43-67
pubmed: 29086303
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4
pubmed: 24753421
Bioinformatics. 2012 Dec 1;28(23):3150-2
pubmed: 23060610
Sci Rep. 2017 Feb 24;7:43232
pubmed: 28233789
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Antimicrob Agents Chemother. 1997 Mar;41(3):563-9
pubmed: 9055993
J Enzyme Inhib Med Chem. 2017 Dec;32(1):917-919
pubmed: 28719998
Nat Methods. 2022 Jun;19(6):679-682
pubmed: 35637307
Nucleic Acids Res. 2022 Jul 5;50(W1):W276-W279
pubmed: 35412617
Nat Biotechnol. 2022 Jul;40(7):1023-1025
pubmed: 34980915
BMC Microbiol. 2019 Jul 30;19(1):174
pubmed: 31362706
Nucleic Acids Res. 2021 Jul 2;49(W1):W293-W296
pubmed: 33885785
Protein Sci. 2021 Jan;30(1):70-82
pubmed: 32881101
J Antimicrob Chemother. 2020 Dec 1;75(12):3491-3500
pubmed: 32780112
Clin Microbiol Rev. 2019 Jul 17;32(4):
pubmed: 31315895
Infection. 2019 Jun;47(3):363-375
pubmed: 30840201
J Med Microbiol. 2012 Jan;61(Pt 1):94-100
pubmed: 21873382
Protein Eng. 1995 Feb;8(2):127-34
pubmed: 7630882
J Clin Microbiol. 2005 Jun;43(6):2551-8
pubmed: 15956362
Biochemistry. 2001 Aug 7;40(31):9207-14
pubmed: 11478888
Bioinformatics. 2014 Jul 15;30(14):2068-9
pubmed: 24642063
Cold Spring Harb Perspect Med. 2016 Aug 01;6(8):
pubmed: 27329032

Auteurs

Johana Becerra (J)

Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil. Electronic address: zbecerra@usp.br.

Gabriel G Araujo (GG)

Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

Felipe Vasquez-Ponce (F)

Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil.

Faride Lamadrid-Feris (F)

Grupo Biodiversidad del Caribe Colombiano, Programa de Biología, Facultad de Ciencias Básicas, Universidad del Atlántico, Barranquilla, Colombia.

Nilton Lincopan (N)

Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil; One Health Brazilian Resistance Project (OneBR), São Paulo, Brazil; Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil. Electronic address: lincopan@usp.br.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › ACT-107, a novel variant of AmpC β-lactamase...
questionsmedicales.fr › Régions géographiques › Amériques › Amérique du Sud › Brésil › ACT-107, a novel variant of AmpC β-lactamase...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Amidohydrolases › bêta-Lactamases › ACT-107, a novel variant of AmpC β-lactamase...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › bêta-Lactames › Céphalosporines › ACT-107, a novel variant of AmpC β-lactamase...