Intracellular Lipid Levels and Oxidative Stress in Peripheral Blood Mononuclear Cells in Experimental Type 1 Diabetes Mellitus.
free fatty acids
lymphocytes
monocytes
reactive oxygen species
type 1 diabetes mellitus
Journal
Bulletin of experimental biology and medicine
ISSN: 1573-8221
Titre abrégé: Bull Exp Biol Med
Pays: United States
ID NLM: 0372557
Informations de publication
Date de publication:
Apr 2023
Apr 2023
Historique:
received:
20
10
2022
medline:
22
5
2023
pubmed:
9
5
2023
entrez:
8
5
2023
Statut:
ppublish
Résumé
Type 1 diabetes mellitus was modeled in Wistar rats by intraperitoneal injection of streptozotocin (25 mg/kg for 5 days), which led to the appearance of the main symptoms of insulin-dependent diabetes. In peripheral blood mononuclear cells isolated by centrifugation on a Ficoll density gradient, the production of ROS and the level of intracellular lipids were evaluated by flow cytofluorimetry. In rats with type 1 diabetes mellitus, an increase in ROS levels in isolated peripheral blood monocytes, but not in the lymphocytic fraction was revealed. Incubation of isolated monocytes in a medium containing 1 mM oleic acid led to a 1.5-fold increase of intracellular lipid levels. After incubation of the lymphocyte fraction in this medium, no differences from the control were revealed. Disorders of carbohydrate and lipid metabolism in type 1 diabetes mellitus leading to an increase of free fatty acids and ROS levels can be detected ex vivo in isolated peripheral blood mononuclear cells.
Identifiants
pubmed: 37157046
doi: 10.1007/s10517-023-05778-2
pii: 10.1007/s10517-023-05778-2
doi:
Substances chimiques
Reactive Oxygen Species
0
Fatty Acids, Nonesterified
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
719-722Informations de copyright
© 2023. Springer Science+Business Media, LLC, part of Springer Nature.
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