Characterization of high-yield Bacillus subtilis cysteine protease for diverse industrial applications.
Cysteine protease
MALDI-TOF
Metal ions
Protease industrial applications
Protease inhibitors
Skimmed milk agar
Journal
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
ISSN: 1678-4405
Titre abrégé: Braz J Microbiol
Pays: Brazil
ID NLM: 101095924
Informations de publication
Date de publication:
Jun 2023
Jun 2023
Historique:
received:
20
10
2022
accepted:
25
04
2023
pmc-release:
09
05
2024
medline:
5
6
2023
pubmed:
9
5
2023
entrez:
8
5
2023
Statut:
ppublish
Résumé
Bacterial proteases have extensive applications in various fields of industrial microbiology. In this study, protease-producing organisms were screened on skimmed milk agar media using serial dilution. Through microbial biomass production, biochemical tests, protease-specific activity, and 16 s RNA gene sequencing, the isolates were identified as Bacillus subtilis and submitted to NCBI. The strain accession numbers were designated as A1 (MT903972), A2 (MT903996), A4 (MT904091), and A5 (MT904796). The strain A4 Bacillus subtilis showed highest protease-specific activity as 76,153.84 U/mg. A4 Bacillus subtilis was unaffected by Ca
Identifiants
pubmed: 37157054
doi: 10.1007/s42770-023-00992-6
pii: 10.1007/s42770-023-00992-6
pmc: PMC10235272
doi:
Substances chimiques
Cysteine Proteases
EC 3.4.-
Peptide Hydrolases
EC 3.4.-
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
739-752Informations de copyright
© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.
Références
J Appl Microbiol. 2020 Jun;128(6):1583-1594
pubmed: 31705597
Biomed Res Int. 2017;2017:8525912
pubmed: 28904973
Appl Microbiol Biotechnol. 2014 Nov;98(21):8853-68
pubmed: 25269600
Crit Rev Biotechnol. 2018 May;38(3):321-334
pubmed: 28789570
Iran J Parasitol. 2020 Apr-Jun;15(2):233-239
pubmed: 32595713
FEMS Microbiol Rev. 2022 Jan 18;46(1):
pubmed: 34410368
Sci Rep. 2019 Jun 25;9(1):9235
pubmed: 31239529
J Struct Biol. 2001 May-Jun;134(2-3):95-102
pubmed: 11551172
Biomed Res Int. 2017;2017:9306564
pubmed: 28785592
N Biotechnol. 2011 Oct;28(6):725-32
pubmed: 21232644
BMC Genomics. 2018 Jan 6;19(1):26
pubmed: 29306330
Sci Rep. 2020 Jun 22;10(1):10033
pubmed: 32572051
Meat Sci. 2004 Nov;68(3):363-9
pubmed: 22062404
3 Biotech. 2021 Oct;11(10):428
pubmed: 34513551
Braz J Microbiol. 2015 Jun 01;46(2):337-46
pubmed: 26273247
Biology (Basel). 2020 Dec 16;9(12):
pubmed: 33339223
3 Biotech. 2019 Nov;9(11):391
pubmed: 31656729
Front Mol Biosci. 2021 May 28;8:680397
pubmed: 34124160
J Gen Physiol. 1938 Sep 20;22(1):79-89
pubmed: 19873094
Bioresour Technol. 2013 Mar;131:76-85
pubmed: 23340105
Polymers (Basel). 2022 Feb 09;14(4):
pubmed: 35215571
Biomolecules. 2013 Nov 08;3(4):923-42
pubmed: 24970197
J Genet Eng Biotechnol. 2021 Apr 6;19(1):53
pubmed: 33825074
J Genet Eng Biotechnol. 2018 Jun;16(1):39-46
pubmed: 30647702
Appl Microbiol Biotechnol. 2017 Nov;101(21):7771-7779
pubmed: 28924866
Front Microbiol. 2020 Aug 04;11:1782
pubmed: 32849401
Sci Rep. 2021 Feb 25;11(1):4573
pubmed: 33633359
Materials (Basel). 2021 Mar 17;14(6):
pubmed: 33803058
Int J Biol Macromol. 2019 May 1;128:254-267
pubmed: 30664968