Transcriptional diversity in specific synaptic gene sets discriminates cortical neuronal identity.
Journal
Biology direct
ISSN: 1745-6150
Titre abrégé: Biol Direct
Pays: England
ID NLM: 101258412
Informations de publication
Date de publication:
09 05 2023
09 05 2023
Historique:
received:
02
03
2023
accepted:
05
04
2023
medline:
11
5
2023
pubmed:
10
5
2023
entrez:
10
5
2023
Statut:
epublish
Résumé
Synapse diversity has been described from different perspectives, ranging from the specific neurotransmitters released, to their diverse biophysical properties and proteome profiles. However, synapse diversity at the transcriptional level has not been systematically identified across all synapse populations in the brain. To quantify and identify specific synaptic features of neuronal cell types we combined the SynGO (Synaptic Gene Ontology) database with single-cell RNA sequencing data of the mouse neocortex. We show that cell types can be discriminated by synaptic genes alone with the same power as all genes. The cell type discriminatory power is not equally distributed across synaptic genes as we could identify functional categories and synaptic compartments with greater cell type specific expression. Synaptic genes, and specific SynGO categories, belonged to three different types of gene modules: gradient expression over all cell types, gradient expression in selected cell types and cell class- or type-specific profiles. This data provides a deeper understanding of synapse diversity in the neocortex and identifies potential markers to selectively identify synapses from specific neuronal populations.
Identifiants
pubmed: 37161421
doi: 10.1186/s13062-023-00372-y
pii: 10.1186/s13062-023-00372-y
pmc: PMC10170694
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
22Investigateurs
Tilmann Achsel
(T)
Maria Andres-Alonso
(M)
Claudia Bagni
(C)
Àlex Bayés
(À)
Thomas Biederer
(T)
Nils Brose
(N)
John Jia En Chua
(JJE)
Marcelo P Coba
(MP)
L Niels Cornelisse
(LN)
Jaime de Juan-Sanz
(J)
Hana L Goldschmidt
(HL)
Eckart D Gundelfinger
(ED)
Richard L Huganir
(RL)
Cordelia Imig
(C)
Reinhard Jahn
(R)
Hwajin Jung
(H)
Pascal S Kaeser
(PS)
Eunjoon Kim
(E)
Frank Koopmans
(F)
Michael R Kreutz
(MR)
Noa Lipstein
(N)
Harold D MacGillavry
(HD)
Peter S McPherson
(PS)
Vincent O'Connor
(V)
Rainer Pielot
(R)
Timothy A Ryan
(TA)
Carlo Sala
(C)
Morgan Sheng
(M)
Karl-Heinz Smalla
(KH)
Paul D Thomas
(PD)
Ruud F Toonen
(RF)
Jan R T van Weering
(JRT)
Chiara Verpelli
(C)
Informations de copyright
© 2023. The Author(s).
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