Sf9 Cell Metabolism Throughout the Recombinant Baculovirus and Rabies Virus-Like Particles Production in Two Culture Systems.
Insect cell metabolism
Rabies virus-like particles
Recombinant baculovirus
Sf9 cells
Stirred tank bioreactor
Viral infection
Journal
Molecular biotechnology
ISSN: 1559-0305
Titre abrégé: Mol Biotechnol
Pays: Switzerland
ID NLM: 9423533
Informations de publication
Date de publication:
10 May 2023
10 May 2023
Historique:
received:
23
09
2022
accepted:
22
04
2023
medline:
10
5
2023
pubmed:
10
5
2023
entrez:
10
5
2023
Statut:
aheadofprint
Résumé
This work aimed to assess the Sf9 cell metabolism during growth, and infection steps with recombinant baculovirus bearing rabies virus proteins, to finally obtain rabies VLP in two culture systems: Schott flask (SF) and stirred tank reactor (STR). Eight assays were performed in SF and STR (four assays in each system) using serum-free SF900 III culture medium. Two non-infection growth kinetics assays and six recombinant baculovirus infection assays. The infection runs were carried out at 0.1 pfu/cell multiplicity of infection (MOI) for single baculovirus bearing rabies glycoprotein (BVG) and matrix protein (BVM) and a coinfection with both baculoviruses at MOI of 3 and 2 pfu/cell for BVG and BVM, respectively. The SF assays were done in triplicate. The glucose, glutamine, glutamate, lactate, and ammonium uptake or release specific rates were quantified over the exponential growth phase and infection stage. The highest uptake specific rate was observed for glucose (42.5 × 10
Identifiants
pubmed: 37162721
doi: 10.1007/s12033-023-00759-2
pii: 10.1007/s12033-023-00759-2
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2018/10538-1
Organisme : Fundação de Amparo à Pesquisa do Estado de São Paulo
ID : 2016/22780-6
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 168539/2018-7
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
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