Role of MicroRNAs and their Downstream Target Transcription Factors in Zebrafish Thrombopoiesis.
Journal
Research square
Titre abrégé: Res Sq
Pays: United States
ID NLM: 101768035
Informations de publication
Date de publication:
24 Apr 2023
24 Apr 2023
Historique:
medline:
10
5
2023
pubmed:
10
5
2023
entrez:
10
5
2023
Statut:
epublish
Résumé
Previous studies have shown that human platelets and megakaryocytes carry microRNAs suggesting their role in platelet function and megakaryocyte development, respectively. However, a comprehensive study on the microRNAs and their targets has not been undertaken. Zebrafish thrombocytes could be used as a model to study their role in megakaryocyte maturation and platelet function because thrombocytes have both megakaryocyte features and platelet properties. In our laboratory, we identified 15 microRNAs in thrombocytes using single-cell RNA sequencing. We knocked down each of these 15 microRNAs by the piggyback method and found knockdown of three microRNAs,
Identifiants
pubmed: 37162944
doi: 10.21203/rs.3.rs-2807790/v1
pmc: PMC10168436
pii:
doi:
Types de publication
Preprint
Langues
eng
Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL159399
Pays : United States
Organisme : NIDDK NIH HHS
ID : R15 DK117384
Pays : United States
Commentaires et corrections
Type : UpdateIn
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