An ultra-sensitive uric acid second generation biosensor based on chemical immobilization of uricase on functionalized multiwall carbon nanotube grafted palm oil fiber in the presence of a ferrocene mediator.

Humans Uric Acid / analysis Urate Oxidase / chemistry Nanotubes, Carbon / chemistry Palm Oil Metallocenes Reproducibility of Results Biosensing Techniques / methods

Journal

Analytical methods : advancing methods and applications
ISSN: 1759-9679
Titre abrégé: Anal Methods
Pays: England
ID NLM: 101519733

Informations de publication

Date de publication:
25 05 2023
Historique:
medline: 26 5 2023
pubmed: 11 5 2023
entrez: 11 5 2023
Statut: epublish

Résumé

In this work, palm oil fiber (POF) grafted functionalized multiwall carbon nanotube (FMWCNT) decorated ferrocene (Fc) has been drop coated on a platinum electrode (Pt), in which uricase (UOx) has been chemically immobilized for sensitive and selective biosensing of uric acid (UA). Through the use of EDC/NHS, a stable bioelectrode (UOx/Fc/FMWCNT-POF/Pt) was obtained and characterized by FTIR/ATRIR, XRD, Raman, EA/EDX, TGA, SEM, TEM, CV, EIS, CA, and DPV. Results from DPV showed the rapid response of the developed bioelectrode towards UA (0.185 V) with high sensitivity (41.14 μA mM

Identifiants

DOI: 10.1039/d3ay00053b PMID: 37165935
pubmed: 37165935
doi: 10.1039/d3ay00053b
doi:

Substances chimiques

Uric Acid 268B43MJ25
Urate Oxidase EC 1.7.3.3
Nanotubes, Carbon 0
Palm Oil 5QUO05548Z
ferrocene U96PKG90JQ
Metallocenes 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2456-2466

Auteurs

Gullit Deffo (G)

Department of Chemical Sciences, Tezpur University, Tezpur, Assam 784028, India. pancha@tezu.ernet.in.
Department of Chemistry, Electrochemistry and Chemistry of Materials, Faculty of Science, University of Dschang, P. O. Box 67, Dschang, Cameroon.

Ranjit Hazarika (R)

Department of Chemical Sciences, Tezpur University, Tezpur, Assam 784028, India. pancha@tezu.ernet.in.

Marcel Cédric Deussi Ngaha (MC)

Department of Chemistry, Electrochemistry and Chemistry of Materials, Faculty of Science, University of Dschang, P. O. Box 67, Dschang, Cameroon.
Department of Chemistry, Higher Teacher Training College, University of Bamenda, Bambili P. O. Box. 39, Cameroon.

Mwina Basumatary (M)

Department of Chemical Sciences, Tezpur University, Tezpur, Assam 784028, India. pancha@tezu.ernet.in.

Shyamali Kalita (S)

Department of Chemical Sciences, Tezpur University, Tezpur, Assam 784028, India. pancha@tezu.ernet.in.

Nayab Hussain (N)

Department of Chemical Sciences, Tezpur University, Tezpur, Assam 784028, India. pancha@tezu.ernet.in.
ORCID: 0000-0001-5113-7988

Evangéline Njanja (E)

Department of Chemistry, Electrochemistry and Chemistry of Materials, Faculty of Science, University of Dschang, P. O. Box 67, Dschang, Cameroon.

Panchanan Puzari (P)

Department of Chemical Sciences, Tezpur University, Tezpur, Assam 784028, India. pancha@tezu.ernet.in.
ORCID: 0000-0002-8043-6677

Emmanuel Ngameni (E)

Department of Chemistry, Laboratory of Analytical Chemistry, University of Yaoundé 1, P.O. Box 812, Yaoundé, Cameroon.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › An ultra-sensitive uric acid second generation...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Purines › Purinones › Xanthines › Acide urique › An ultra-sensitive uric acid second generation...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Oxidoreductases › Urate oxidase › An ultra-sensitive uric acid second generation...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Nanostructures › Nanotubes › Nanotubes de carbone › An ultra-sensitive uric acid second generation...
questionsmedicales.fr › Mélanges complexes › Produits biologiques › Préparations à base de plantes › Huiles végétales › Huile de palme › An ultra-sensitive uric acid second generation...
questionsmedicales.fr › Composés chimiques organiques › Composés organométalliques › Métallocènes › An ultra-sensitive uric acid second generation...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › An ultra-sensitive uric acid second generation...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › An ultra-sensitive uric acid second generation...