Single-Cell Glycan and RNA Sequencing (scGR-seq).

Sequence Analysis, RNA Cell Membrane / metabolism Polysaccharides / chemistry Lectins / chemistry RNA / genetics
RNA glycan lectin sequencing single-cell

Journal

Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894

Informations de publication

Date de publication:
May 2023
Historique:
medline: 15 5 2023
pubmed: 11 5 2023
entrez: 11 5 2023
Statut: ppublish

Résumé

Single-cell multi-omics analysis has emerged as a crucial tool in modern biology due to its capacity to uncover the intricate molecular heterogeneity within individual cells. Glycan structural diversity on the cell surface creates a "cell signature" that varies by cell type and state. However, single-cell glycan analysis remains a challenge. This protocol presents a series of techniques and procedures that enable the simultaneous measurements of glycan and RNA at the single-cell level via DNA-barcoded lectin-based sequencing (scGR-seq). The techniques include (1) the preparation of DNA-barcoded lectins, (2) a step-by-step protocol for single-cell glycan/RNA sequencing, and (3) a data analysis approach for the integration of glycan and RNA data. © 2023 Wiley Periodicals LLC. Basic Protocol 1: Preparation of DNA-barcoded lectins Basic Protocol 2: Single-cell glycan/RNA sequencing.

Identifiants

DOI: 10.1002/cpz1.777 PMID: 37166286
pubmed: 37166286
doi: 10.1002/cpz1.777
doi:

Substances chimiques

Polysaccharides 0
Lectins 0
RNA 63231-63-0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e777

Subventions

Organisme : JST PRESTO
ID : JPMJPR16F6
Organisme : JST A-STEP
ID : JPMJTR20UD
Organisme : MEXT/JSPS KAKENHI
ID : 22K19471

Informations de copyright

© 2023 Wiley Periodicals LLC.

Auteurs

Haruki Odaka (H)

Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.

Hiroaki Tateno (H)

Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
JST PRESTO, Tsukuba, Ibaraki, Japan.

Articles similaires

Exploring transcriptomic mechanisms underlying pulmonary adaptation to diverse environments in Indian rams.

Ritika Gera, Reena Arora, Pooja Chhabra et al.
1.00
Animals Lung India Sheep Transcriptome

Biological functions and molecular mechanisms of exosome-derived circular RNAs and their clinical implications in digestive malignancies: the vintage in the bottle.

Yuanyan Lou, Jianing Yan, Qingqing Liu et al.
1.00
Humans RNA, Circular Exosomes Cell Proliferation Epithelial-Mesenchymal Transition

Dietary supplementation with xylanase suppresses the antinutritional effect of nonstarch polysaccharides of flaxseed and increases bone strength in broiler chickens.

Miloš Skřivan, Michaela Englmaierová, Milan Marounek et al.
1.00
Animals Flax Chickens Dietary Supplements Endo-1,4-beta Xylanases

Cell-cell junctions in focus - imaging junctional architectures and dynamics at high resolution.

Vera Janssen, Stephan Huveneers
1.00
Humans Animals Adherens Junctions Intercellular Junctions Tight Junctions

Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Analyse de séquence d'ARN › Single-Cell Glycan and RNA Sequencing (scGR-seq).
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Single-Cell Glycan and RNA Sequencing (scGR-seq).
questionsmedicales.fr › Glucides › Polyosides › Single-Cell Glycan and RNA Sequencing (scGR-seq).
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Lectines › Single-Cell Glycan and RNA Sequencing (scGR-seq).
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › Single-Cell Glycan and RNA Sequencing (scGR-seq).