Mechanisms of skin vascular maturation and maintenance captured by longitudinal imaging of live mice.
angiogenesis
blood flow
capillary
endothelial cell
live imaging
neonatal development
skin
vascular homeostasis
vascular repair
vessel regression
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
25 05 2023
25 05 2023
Historique:
received:
01
12
2021
revised:
03
02
2023
accepted:
11
04
2023
pmc-release:
25
05
2024
medline:
29
5
2023
pubmed:
12
5
2023
entrez:
11
5
2023
Statut:
ppublish
Résumé
A functional network of blood vessels is essential for organ growth and homeostasis, yet how the vasculature matures and maintains homeostasis remains elusive in live mice. By longitudinally tracking the same neonatal endothelial cells (ECs) over days to weeks, we found that capillary plexus expansion is driven by vessel regression to optimize network perfusion. Neonatal ECs rearrange positions to evenly distribute throughout the developing plexus and become positionally stable in adulthood. Upon local ablation, adult ECs survive through a plasmalemmal self-repair response, while neonatal ECs are predisposed to die. Furthermore, adult ECs reactivate migration to assist vessel repair. Global ablation reveals coordinated maintenance of the adult vascular architecture that allows for eventual network recovery. Lastly, neonatal remodeling and adult maintenance of the skin vascular plexus are orchestrated by temporally restricted, neonatal VEGFR2 signaling. Our work sheds light on fundamental mechanisms that underlie both vascular maturation and adult homeostasis in vivo.
Identifiants
pubmed: 37167971
pii: S0092-8674(23)00413-0
doi: 10.1016/j.cell.2023.04.017
pmc: PMC10225355
mid: NIHMS1893073
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2345-2360.e16Subventions
Organisme : NIA NIH HHS
ID : DP1 AG066590
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR067755
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK118728
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR072668
Pays : United States
Organisme : NIBIB NIH HHS
ID : UH2 EB017103
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL146056
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR063663
Pays : United States
Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing financial interests.
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